Abstract

Long non-coding RNAs (lncRNAs) are involved in many cellular processes while displaying high tissue specificity. In contrast, protein-coding genes, including the category of housekeeping ones, exhibit broad expression patterns. The aim of this study was to highlight the functional importance of widely expressed lncRNAs. We analyzed experimental data from cell-growth screen of lncRNA loci in human cells, which allowed us to identify 18 lncRNA hits. Notably, these lncRNAs were not only widely expressed in most human tissues, but also played functional roles within them. Detail investigation revealed them encompass a variety of molecular functions, from cardiomyocyte damage controlling to macrophage class switching. Interestingly, experimental data highlighted the fact that a significant part of these lncRNAs encoded small but functional polypeptides. A set of lncRNAs, NEAT1, SNHG1, SNHG7, SNHG12, SNHG15, SNHG16, MIR17HG, LINC00680, LINC00263 and LINC00339, that were highly likely to be translated into small polypeptides was identified. Additionally, for EPB41L4A-AS1, CRNDE, SNHG6, LINC00493, and LINC01420, a dual function associated with both the RNA sequences and small proteins they encoded was established.

中文翻译：

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功能相关且广泛表达的长非编码 RNA 的修订

摘要

长非编码 RNA (lncRNA) 参与许多细胞过程，同时表现出高组织特异性。相比之下，蛋白质编码基因，包括管家基因，表现出广泛的表达模式。本研究的目的是强调广泛表达的 lncRNA 的功能重要性。我们分析了人类细胞中 lncRNA 位点细胞生长筛选的实验数据，这使我们能够识别 18 个 lncRNA 命中。值得注意的是，这些lncRNA不仅在大多数人体组织中广泛表达，而且在其中发挥功能作用。详细研究表明它们包含多种分子功能，从心肌细胞损伤控制到巨噬细胞类别转换。有趣的是，实验数据强调了这样一个事实：这些 lncRNA 的很大一部分编码小但有功能的多肽。鉴定出一组极有可能被翻译成小多肽的lncRNA：NEAT1、SNHG1、SNHG7、SNHG12、SNHG15、SNHG16、MIR17HG、LINC00680、LINC00263和LINC00339。此外，对于 EPB41L4A-AS1、CRNDE、SNHG6、LINC00493 和 LINC01420，建立了与 RNA 序列和它们编码的小蛋白质相关的双重功能。