Humans and their immune system are confronted with mold-contaminated food and/or mold-contaminated air in daily life and indoor activities. This results in metabolic stress and unspecific disease symptoms. Other studies provided evidence that exposure to mold is associated with the etiology of allergies. Deoxynivalenol (DON) is of great concern due to its frequent occurrence in toxically relevant concentrations. The exposure to this toxin is a permanent health risk for both humans and farm animals because DON cannot be significantly removed during standard milling and processing procedures. However, the direct effect on immunity or hematology is poorly defined because most investigations could not separate the effect of DON-contaminated feed intake. Due to the widespread distribution of DON after rapid absorption, it is not surprising that DON is known to affect the immune system. The immune system of the organism has one important function, to defend against the invasion of unknown substances/organisms. This study shows for the first time a synergistic effect of both—low physiological DON-doses in combination with low LPS-doses with the focus on the IL-8 expression on protein and RNA level. Both doses were found in vivo. IL-8 together with other anorectic cytokines like IL-1β can affect the food intake and anorexia. We could also show that a calcium-response is not involved in the increased IL-8 production after acute DON stimulation with high or low concentrations.

人类及其免疫系统在日常生活和室内活动中面临着受霉菌污染的食物和/或受霉菌污染的空气。这会导致代谢应激和非特异性疾病症状。其他研究提供的证据表明，接触霉菌与过敏的病因有关。脱氧雪腐镰刀菌烯醇 (DON) 因其在毒性相关浓度下的频繁出现而备受关注。接触这种毒素对人类和农场动物都会造成永久性的健康风险，因为在标准研磨和加工过程中无法显着去除呕吐毒素。然而，对免疫或血液学的直接影响尚不清楚，因为大多数研究无法区分受 DON 污染的采食量的影响。由于 DON 快速吸收后分布广泛，因此 DON 影响免疫系统也就不足为奇了。机体的免疫系统有一项重要功能，就是防御未知物质/生物体的入侵。这项研究首次显示了低生理 DON 剂量与低 LPS 剂量相结合的协同效应，重点关注蛋白质和 RNA 水平上的 IL-8 表达。两种剂量均在体内发现。IL-8 与其他厌食细胞因子（如 IL-1β）一起可以影响食物摄入和厌食。我们还可以证明，在高浓度或低浓度的急性 DON 刺激后，钙反应并不参与 IL-8 产生的增加。