Paper-based electrochemical sensors have the characteristics of flexibility, biocompatibility, environmental protection, low cost, wide availability, and hydropathy, which make them very suitable for the development and application of biological detection. This work proposes electrospun cellulose acetate nanofiber (CA NF)-decorated paper-based screen-printed (PBSP) electrode electrochemical sensors. The CA NFs were directly collected on the PBSP electrode through an electrospinning technique at an optimized voltage of 16 kV for 10 min. The sensor was functionalized with different bio-sensitive materials for detecting different targets, and its sensing capability was evaluated by CV, DPV, and chronoamperometry methods. The test results demonstrated that the CA NFs enhanced the detection sensitivity of the PBSP electrode, and the sensor showed good stability, repeatability, and specificity (p < 0.01, N = 3). The electrochemical sensing of the CA NF-decorated PBSP electrode exhibited a short detection duration of ∼5–7 min and detection ranges of 1 nmol mL⁻¹–100 μmol mL⁻¹, 100 fg mL⁻¹–10 μg mL⁻¹, and 1.5 × 10²–10⁶ CFU mL⁻¹ and limits of detection of 0.71 nmol mL⁻¹, 89.1 fg mL⁻¹, and 30 CFU mL⁻¹ for glucose, Ag85B protein, and E. coli O157:H7, respectively. These CA NF-decorated PBSP sensors can be used as a general electrochemical tool to detect, for example, organic substances, proteins, and bacteria, which are expected to achieve point-of-care testing of pathogenic microorganisms and have wide application prospects in biomedicine, clinical diagnosis, environmental monitoring, and food safety.

中文翻译：

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由电纺醋酸纤维素纳米纤维装饰的一次性纸基电化学生物传感器，用于高灵敏度生物检测

纸基电化学传感器具有柔性、生物相容性、环保、成本低、可用性广泛、亲水等特点，非常适合生物检测的开发和应用。这项工作提出了电纺醋酸纤维素纳米纤维（CA NF）装饰的纸基丝网印刷（PBSP）电极电化学传感器。通过静电纺丝技术，在优化电压 16 kV 下 10 分钟，将 CA NF 直接收集在 PBSP 电极上。该传感器采用不同的生物敏感材料进行功能化，以检测不同的目标，并通过CV、DPV和计时电流法评估其传感能力。测试结果表明，CA NFs增强了PBSP电极的检测灵敏度，传感器表现出良好的稳定性、重复性和特异性（p < 0.01，N = 3）。CA NF修饰的PBSP电极的电化学传感表现出~5-7分钟的短检测时间和1 nmol mL ^-1 –100 μmol mL ^-1、100 fg mL ^-1 –10 μg mL ^-1的检测范围，葡萄糖、Ag85B 蛋白和大肠杆菌 O157:H7 的检测限分别为 1.5 × 10 ² –10 ⁶ CFU mL ^-1和 1.5 × 10 2 –10 6 CFU mL ^-1 ，检测限分别为 0.71 nmol mL ^{-1 、89.1 fg mL -1}和 30 CFU mL -1 。这些CA NF修饰的PBSP传感器可作为通用电化学工具来检测有机物、蛋白质和细菌等，有望实现病原微生物的即时检测，在生物医学领域具有广泛的应用前景、临床诊断、环境监测和食品安全。