An important regulatory role for ethylene-responsive transcription factors (ERFs) is in plant growth and development, stress response, and hormone signaling. However, AP2/ERF family genes in mango have not been systematically studied. In this study, a total of 113 AP2/ERF family genes were identified from the mango genome and phylogenetically classified into five subfamilies: AP2 (28 genes), DREB (42 genes), ERF (33 genes), RAV (6 genes), and Soloist (4 genes). Of these, the ERF family, in conjunction with Arabidopsis and rice, forms a phylogenetic tree divided into seven groups, five of which have MiERF members. Analysis of gene structure and -elements showed that each MiERF gene contains only one AP2 structural domain, and that MiERF genes contain a large number of -elements associated with hormone signaling and stress response. Collinearity tests revealed a high degree of homology between s and s. Tissue-specific and stress-responsive expression profiling revealed that MiERF genes are primarily involved in the regulation of reproductive growth and are differentially and positively expressed in response to external hormones and pathogenic bacteria. Physiological results from a gain-of-function analysis of transiently overexpressed in tobacco and mango showed that transient expression of resulted in decreased colony count and callose deposition, as well as varying degrees of response to hormonal signals such as ETH, JA, and SA. Thus, may be involved in the JA/ETH signaling pathway to enhance plant defense against pathogenic bacteria. This study provides a basis for further research on the function and regulation of MiERF genes and lays a foundation for the selection of disease-resistant genes in mango.

中文翻译：

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芒果ERF基因家族的鉴定及MiERF4对野油菜黄单胞菌的防御反应。芒果

乙烯响应转录因子 (ERF) 在植物生长和发育、应激反应和激素信号传导中发挥着重要的调节作用。然而，芒果中的AP2/ERF家族基因尚未得到系统研究。本研究从芒果基因组中鉴定出总共113个AP2/ERF家族基因，并根据系统发育分为5个亚家族：AP2（28个基因）、DREB（42个基因）、ERF（33个基因）、RAV（6个基因）、和独奏者（4 个基因）。其中，ERF家族与拟南芥和水稻一起形成系统发育树，分为七个类群，其中五个类群有MiERF成员。基因结构和β元件分析表明，每个MiERF基因只含有一个AP2结构域，而MiERF基因含有大量与激素信号传导和应激反应相关的β元件。共线性检验显示 s 和 s 之间具有高度的同源性。组织特异性和应激反应性表达谱表明，MiERF 基因主要参与生殖生长的调节，并且在响应外部激素和病原菌时差异性地积极表达。在烟草和芒果中瞬时过表达的功能获得分析的生理学结果表明，瞬时表达导致菌落计数和胼胝质沉积减少，以及对 ETH、JA 和 SA 等激素信号的不同程度的反应。因此，可能参与 JA/ETH 信号通路以增强植物对病原菌的防御。本研究为进一步研究MiERF基因的功能和调控奠定了基础，并为芒果抗病基因的筛选奠定了基础。