In order to study the responses of tomato () WRKY TFs to bacterial wilt caused by , the most up-to-date genomes and transcriptional profiles were used to identify WRKY TFs in control and infected inbred lines. In total, 85 tomato WRKY TFs were identified and categorized into groups I, IIa + b, IIc, IId + e, and III. These WRKYs, especially those from group IIe, were mainly distributed at chromosome ends and in clusters. More than 45 % and 70 % of tomato WRKYs exhibited intraspecific and interspecific synteny, respectively. Nearly 60 % of tomato WRKYs (mainly in groups I and IIc) formed 73 pairs of orthologs with WRKYs in Arabidopsis and pepper, with Ka/Ks less than 1. Sixteen tomato WRKYs (mainly in groups IIa + b and IIc) responded strongly to biotic stress, and 12 differentially expressed WRKYs (mainly in groups III and IIb) were identified. RT-qPCR revealed that tomato WRKYs could respond to bacterial wilt through positive (predominant) or negative regulation. In particular, the interaction between Solyc03g095770.3 (group III) and Solyc09g014990.4 (group I) may play an important role. In brief, WRKY TFs were comprehensively identified in tomato and several bacterial wilt responsive genes were screened.

中文翻译：

---

番茄 WRKY 转录因子的比较鉴定和对青枯菌的转录反应

为了研究番茄 () WRKY TF 对青枯病引起的反应，使用最新的基因组和转录谱来鉴定对照和感染自交系中的 WRKY TF。总共鉴定了 85 个番茄 WRKY TF，并将其分为 I、IIa + b、IIc、IId + e 和 III 组。这些WRKY，尤其是来自IIe组的WRKY，主要分布在染色体末端并成簇分布。超过 45% 和 70% 的番茄 WRKY 分别表现出种内和种间同线性。近60%的番茄WRKY（主要在I组和IIc组）与拟南芥和辣椒中的WRKY形成73对直系同源物，Ka/Ks小于1。16个番茄WRKY（主要在IIa + b组和IIc组）对生物胁迫，并鉴定出 12 个差异表达的 WRKY（主要在 III 组和 IIb 组）。 RT-qPCR 显示番茄 WRKY 可以通过正向（主要）或负向调节对青枯病作出反应。特别是，Solyc03g095770.3（第 III 组）和 Solyc09g014990.4（第 I 组）之间的相互作用可能发挥重要作用。总之，在番茄中全面鉴定了WRKY转录因子，并筛选了几个青枯病响应基因。