is one of the important tree species in southwest China, and it has significant economic and ecological value. However, there is still a lack of effective methods to identify the functional genes of By verifying the model plant tobacco, the pTRV2::JsPDS vector was able to cause photobleaching. This study showed that photobleaching occurred 24 and 30 d after the silencing vector was infected with aseptic seedlings and fruits of , respectively. When the OD was 0.6, and the injection dose was 500 μL, the gene silencing efficiency of aseptic seedlings was the highest at 16.7 %, significantly better than other treatments. Moreover, when the OD was 0.8, and the injection dose was 500 μL, the gene silencing efficiency in the walnut fruit was the highest (20 %). In addition, the VIGS system was successfully used to silence and genes in . This study also showed that the flavonol content and gene expression in the treatment group were decreased compared to the control group. In addition, the proteins transcribed and translated from the gene may have higher catalytic activity for dihydroquercetin. The above results indicate that the TRV-mediated VIGS system can be an ideal tool for studying gene function.

中文翻译：

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核桃病毒诱导基因沉默系统的建立及JsFLS2和JsFLS4的功能分析

是西南地区重要树种之一，具有重要的经济和生态价值。然而，目前仍缺乏有效的方法来鉴定pTRV2::JsPDS载体的功能基因。通过验证模型植物烟草，pTRV2::JsPDS载体能够引起光漂白。本研究表明，沉默载体分别感染无菌幼苗和果实后24和30天发生光漂白。当OD为0.6、注射剂量为500μL时，无菌苗的基因沉默效率最高，为16.7%，显着优于其他处理。而且，当OD为0.8、注射剂量为500μL时，核桃果实中的基因沉默效率最高（20%）。此外，VIGS系统已成功用于沉默和基因。本研究还表明，与对照组相比，治疗组的黄酮醇含量和基因表达量有所降低。此外，该基因转录和翻译的蛋白质可能对二氢槲皮素具有更高的催化活性。上述结果表明TRV介导的VIGS系统可以成为研究基因功能的理想工具。