The ChaC1 enzyme that catalyzes cytosolic glutathione degradation is highly upregulated in several cancers. In a systematic review of gene signature panels for cancer prognosis based on oxidative stress and ferroptosis genes, we observed that ChaC1 was found in panels in a wide variety of different cancers, with the upregulation correlating with poor prognosis. Since SNPs can have an impact on functionality and prognosis, ChaC1 SNPs from various databases were also investigated. Six frequently observed missense SNPs were chosen for reconstruction, and their functionality was evaluated. Three out of six SNPs resulted in either a partial or complete loss of ChaC1 function, and these SNPs had the changes R72Q, A156V, and G173S in their proteins. This study highlights the importance of ChaC1 in cancer prognosis across a wide variety of cancers. Additionally, the information on the SNPs of ChaC1 with altered enzymatic activities would improve the prognostic ability of these panels and facilitate treatment regimens.

中文翻译：

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ChaC1 上调反映了多种癌症的不良预后：分析 ChaC1 的主要错义 SNP 有助于改善预后

催化胞质谷胱甘肽降解的 ChaC1 酶在多种癌症中高度上调。在对基于氧化应激和铁死亡基因的癌症预后基因特征组进行系统评价时，我们观察到 ChaC1 存在于多种不同癌症的基因组中，其上调与不良预后相关。由于 SNP 可能对功能和预后产生影响，因此还对来自不同数据库的 ChaC1 SNP 进行了研究。选择六个经常观察到的错义 SNP 进行重建，并评估其功能。六个 SNP 中的三个导致 ChaC1 功能部分或完全丧失，这些 SNP 的蛋白质发生了 R72Q、A156V 和 G173S 的变化。这项研究强调了 ChaC1 在多种癌症的癌症预后中的重要性。此外，有关酶活性改变的 ChaC1 SNP 的信息将提高这些组的预后能力并促进治疗方案。