is widely associated with biofilm-mediated antibiotic resistant chronic and acute infections which constitute a persistent healthcare challenges. Addressing this threat requires exploration of novel therapeutic strategies involving the combination of natural compounds and conventional antibiotics. Hence, our study has focused on two compounds; cuminaldehyde and ciprofloxacin, which were strategically combined to target the biofilm challenge of . The minimum inhibitory concentration (MIC) of cuminaldehyde and ciprofloxacin was found to be 400 μg/mL and 0.4 μg/mL, respectively. Moreover, the fractional inhibitory concentration index (FICI = 0.62) indicated an additive interaction prevailed between cuminaldehyde and ciprofloxacin. Subsequently, sub-MIC doses of cuminaldehyde (25 μg/mL) and ciprofloxacin (0.05 μg/mL) were selected for an array of antibiofilm assays which confirmed their biofilm inhibitory potential without exhibiting any antimicrobial activity. Furthermore, selected doses of the mentioned compounds could manage biofilm on catheter surface by inhibiting and disintegrating existing biofilm. Additionally, the test combination of the mentioned compounds reduced virulence factors secretion, accumulated reactive oxygen species and increased cell-membrane permeability. Thus, the combination of cuminaldehyde and ciprofloxacin demonstrates potential in combating biofilm-associated Pseudomonal threats.

中文翻译：

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应用异丙苯醛和环丙沙星有效控制铜绿假单胞菌生物膜组装：一项组合研究

与生物膜介导的抗生素耐药性慢性和急性感染广泛相关，这构成了持续的医疗保健挑战。应对这一威胁需要探索涉及天然化合物和常规抗生素组合的新型治疗策略。因此，我们的研究重点关注两种化合物：对异丙苯醛和环丙沙星进行战略性组合，以应对 .异丙苯醛和环丙沙星的最低抑菌浓度 (MIC) 分别为 400 μg/mL 和 0.4 μg/mL。此外，分数抑制浓度指数（FICI = 0.62）表明异丙苯醛和环丙沙星之间存在加成相互作用。随后，选择亚 MIC 剂量的异丙苯醛 (25 μg/mL) 和环丙沙星 (0.05 μg/mL) 进行一系列抗生物膜测定，证实了它们的生物膜抑制潜力，但不表现出任何抗菌活性。此外，选定剂量的上述化合物可以通过抑制和分解现有的生物膜来管理导管表面的生物膜。此外，上述化合物的测试组合减少了毒力因子的分泌，积累了活性氧并增加了细胞膜的通透性。因此，异丙苯醛和环丙沙星的组合显示出对抗生物膜相关假单胞菌威胁的潜力。