The Cluster of Differentiation 27 (CD27) is aberrantly expressed in multiple myeloma (MM) -derived. This expression facilitates the interaction between tumor and immune cells within TME via the CD27-CD70 pathway, resulting in immune evasion and subsequent tumor progression. The objective of this study is to investigate the correlation between CD27 expression and the prognosis of MM, and to elucidate its potential relationship with the immune microenvironment. In this research, CD27 expression in T cells within the 82 newly diagnosed MM microenvironment was assessed via flow cytometry. We then examined the association between CD27 expression levels and patient survival. Subsequent a series of bioinformatics and in vitro experiments were conducted to reveal the role of CD27 in MM. Clinical evidence suggests that elevated CD27 expression in T cells within the bone marrow serves as a negative prognostic marker for MM survival. Data analysis from the GEO database has demonstrated a strong association between MM-derived CD27 and the immune response, as well as the hematopoietic system. Importantly, patients with elevated levels of CD27 expression were also found to have an increased presence of MDSCs and macrophages in the bone marrow microenvironment. Furthermore, the PERK-ATF4 signaling pathway has been implicated in mediating the effects of CD27 in MM. We revealed that CD27 expression levels serve as an indicative marker for the prognosis of MM patients. The CD27- PERK-ATF4 is a promising target for the treatment of MM.

中文翻译：

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多发性骨髓瘤的预后标志物CD27及其微环境

分化簇 27 (CD27) 在多发性骨髓瘤 (MM) 来源中异常表达。这种表达促进 TME 内肿瘤和免疫细胞之间通过 CD27-CD70 途径相互作用，导致免疫逃避和随后的肿瘤进展。本研究的目的是探讨CD27表达与MM预后的相关性，并阐明其与免疫微环境的潜在关系。在这项研究中，通过流式细胞术评估了 82 个新诊断的 MM 微环境中 T 细胞的 CD27 表达。然后我们检查了 CD27 表达水平与患者生存率之间的关联。随后进行了一系列生物信息学和体外实验来揭示CD27在MM中的作用。临床证据表明，骨髓内 T 细胞 CD27 表达升高是 MM 生存的负面预后标志。GEO 数据库的数据分析表明，MM 衍生的 CD27 与免疫反应以及造血系统之间存在密切关联。重要的是，CD27 表达水平升高的患者还发现骨髓微环境中 MDSC 和巨噬细胞的存在增加。此外，PERK-ATF4 信号通路参与介导 CD27 在 MM 中的作用。我们发现 CD27 表达水平可作为 MM 患者预后的指示性标志物。CD27-PERK-ATF4 是治疗 MM 的一个有希望的靶点。