Fully integrated devices that enable full functioning execution without or with minimum external accessories or equipment are deemed to be one of the most desirable and ultimate objectives for modern device design and construction. Escherichia coli O157:H7 (E. coli O157:H7) is often linked to outbreaks caused by contaminated water and food. However, the sensors that are currently used for point-of-care E. coli O157:H7 (E. coli O157:H7) detection are often large and cumbersome. Herein, we demonstrate the first example of a handheld and pump-free fully integrated electrochemical sensing platform with the capability to point-of-care test E. coli O157:H7 in the actual samples of E. coli O157:H7-spiked tap water and E. coli O157:H7-spiked watermelon juice. This platform was made possible by overcoming major engineering challenges in the seamless integration of a microfluidic module for pump-free liquid sample collection and transportation, a sensing module for efficient E. coli O157:H7 testing, and an electronic module for automatically converting and wirelessly transmitting signals into a single and compact electrochemical sensing platform that retains its inimitable stand-alone, handheld, pump-free, and cost-effective feature. Although our primary emphasis in this study is on detecting E. coli O157:H7, this pump-free fully integrated handheld electrochemical sensing platform may also be used to monitor other pathogens in food and water by including specific antipathogen antibodies.

中文翻译：

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用于大肠杆菌 O157:H7 即时检测的完全集成手持式电化学传感平台

完全集成的设备无需或只需最少的外部附件或设备即可实现全功能执行，被认为是现代设备设计和构造最理想和最终的目标之一。大肠杆菌O157:H7 ( E. coli O157:H7) 通常与受污染的水和食物引起的疫情有关。然而，目前用于即时检测大肠杆菌O157:H7 ( E. coli O157:H7) 的传感器通常又大又笨重。在此，我们展示了手持式、无泵完全集成电化学传感平台的第一个示例，该平台能够在添加了大肠杆菌O157:H7的自来水的实际样品中进行即时检测大肠杆菌O157:H7以及添加了大肠杆菌O157:H7 的西瓜汁。该平台的实现是通过克服无缝集成用于无泵液体样品收集和运输的微流体模块、用于高效大肠杆菌O157:H7 测试的传感模块以及用于自动转换和无线传输的电子模块的主要工程挑战而实现的。将信号传输到单个紧凑的电化学传感平台，该平台保留了其独特的独立、手持、无泵和成本效益的特性。尽管我们在本研究中的主要重点是检测大肠杆菌O157:H7，但这种无泵完全集成手持式电化学传感平台也可通过包含特定的抗病原体抗体来监测食品和水中的其他病原体。