The oriental tobacco budworm (Lepidoptera: Noctuidae) is a specialist pest that may cause serious damages to important crops such as chili pepper and tobacco. Various man-made insecticides have been applied to control the infestation of this pest. To understand how this pest copes with insecticides, it is required to identify key players involved in insecticide transformation. In this study, a P450 gene of CYP6B subfamily was identified in the oriental tobacco budworm, and its expression pattern was revealed Moreover, the activities of HassCYP6B6 against 12 insecticides were explored using recombinant enzymes produced in the facile . Data from metabolic experiments showed that HassCYP6B6 was able to metabolize conventional insecticides including organophosporates (diazinon, malathion, phoxim), carbamate propoxur, and pyrethroid esfenvalerate, while no significant metabolism was observed towards new-type pesticides such as neonicotinoids (acetamiprid, imidacloprid), diamides (chlorantraniliprole, cyantraniliprole), macrocyclic lactone (emamectin benzoate, ivermectin), and metaflumizone. Structures of metabolites were proposed based on mass spectrometry analyses. The results demonstrate that HassCYP6B6 plays important roles in the transformation of multiple insecticides via substrate-dependent catalytic mechanisms including dehydrogenation, hydroxylation and oxidative desulfurization. The findings have important applied implications for the usage of insecticides.

中文翻译：

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Helicoverpa assulta CYP6B6 在杀虫剂代谢中的功能表征

烟草芽虫（鳞翅目：夜蛾科）是一种特殊害虫，可能对辣椒和烟草等重要作物造成严重损害。已使用各种人造杀虫剂来控制这种害虫的侵扰。为了了解这种害虫如何应对杀虫剂，需要确定参与杀虫剂转化的关键参与者。在本研究中，在香料烟芽虫中鉴定了CYP6B亚家族的P450基因，并揭示了其表达模式。此外，利用简便产生的重组酶探索了HassCYP6B6对12种杀虫剂的活性。代谢实验数据表明，HassCYP6B6能够代谢传统杀虫剂，包括有机磷农药（二嗪磷、马拉硫磷、辛硫磷）、氨基甲酸酯残杀威、拟除虫菊酯高氰戊菊酯，而对新烟碱类农药（啶虫脒、吡虫啉）、二酰胺（氯虫苯甲酰胺、氰虫苯甲酰胺）、大环内酯（甲维菌素苯甲酸盐、伊维菌素）和氰氟虫胺。根据质谱分析提出了代谢物的结构。结果表明，HassCYP6B6 通过底物依赖性催化机制（包括脱氢、羟基化和氧化脱硫）在多种杀虫剂的转化中发挥重要作用。这些发现对杀虫剂的使用具有重要的应用意义。