The whitefly poses a significant threat to various crops and ornamental plants and causes severe damage to the agricultural industry. Over the past few decades, has developed resistance to several pesticides, including imidacloprid. Therefore, elucidating the mechanism that leads to insecticide detoxification is very important for controlling and managing whitefly resistance to neonicotinoid insecticides Among insect detoxification enzymes, glutathione S-transferase (GST) is an important phase II detoxification enzyme that helps detoxify exogenous toxic substances. In this study, we cloned the gene and observed that its expression level was greater in imidacloprid-resistant populations than sensitive populations of . By silencing via RNA interference, we found a significant increase in the mortality of imidacloprid-resistant . Additionally, prokaryotic expression and in vitro metabolism studies revealed that the recombinant protein could metabolize 36.36% of the total imidacloprid, providing direct evidence that plays a crucial role in the detoxification of imidacloprid. Overall, our study elucidated the role of GSTs in physiological activities related to insecticide resistance, which helps clarify the resistance mechanisms conferred by GSTs and provides useful insights for sustainable integrated pest management.

中文翻译：

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谷胱甘肽 S-转移酶直接代谢烟粉虱体内的吡虫啉

白粉虱对多种农作物和观赏植物构成重大威胁，并对农业造成严重损害。在过去的几十年里，已经对几种农药产生了抗药性，包括吡虫啉。因此，阐明杀虫剂解毒机制对于控制和管理烟粉虱对新烟碱类杀虫剂的抗性非常重要。在昆虫解毒酶中，谷胱甘肽S-转移酶（GST）是一种重要的II相解毒酶，有助于解毒外源有毒物质。在这项研究中，我们克隆了该基因，并观察到其在吡虫啉耐药群体中的表达水平高于敏感群体。通过 RNA 干扰沉默，我们发现吡虫啉耐药性的死亡率显着增加。此外，原核表达和体外代谢研究表明，重组蛋白可代谢吡虫啉总量的36.36%，为吡虫啉解毒发挥重要作用提供了直接证据。总体而言，我们的研究阐明了 GST 在与杀虫剂抗性相关的生理活动中的作用，这有助于阐明 GST 赋予的抗性机制，并为可持续综合害虫管理提供有用的见解。