Insect pests cause immense agronomic losses worldwide. One of the most destructive of major crops is the Fall Armyworm (, FAW). The ability to migrate long distances, a prodigious appetite, and a demonstrated ability to develop resistance to insecticides, make it a difficult target to control. Insecticidal proteins, for example those produced by the bacterium , are among the safest and most effective insect control agents. Genetically modified (GM) crops expressing such proteins are a key part of a successful integrated pest management (IPM) program for FAW. However, due to the development of populations resistant to commercialized GM products, new GM traits are desperately needed. Herein, we describe a further characterization of the newly engineered trait protein eCry1Gb.1Ig. Similar to other well characterized Cry proteins, eCry1Gb.1Ig is shown to bind FAW midgut cells and induce cell-death. Binding competition assays using trait proteins from other FAW-active events show a lack of competition when binding FAW brush border membrane vesicles (BBMVs) and when utilizing non-pore-forming versions as competitors in bioassays. Similarly, insect cell lines expressing 2 and 3 (well characterized receptors of existing commercial Cry proteins) are insensitive to eCry1Gb.1Ig. These findings are consistent with results from our previous work showing that eCry1Gb.1Ig is effective in controlling insects with resistance to existing traits. This underscores the value of eCry1Gb.1Ig as a new GM trait protein with a unique site-of-action and its potential positive impact to global food production.

中文翻译：

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eCry1Gb.1Ig 的作用模式的表征，eCry1Gb.1Ig 是一种草地贪夜蛾 (Spodoptera frugiperda) 活性蛋白，具有新的作用位点

害虫在全世界造成巨大的农业损失。最具破坏性的主要农作物之一是秋粘虫（FAW）。长距离迁徙的能力、巨大的食欲以及对杀虫剂产生抗性的能力，使其成为难以控制的目标。杀虫蛋白，例如由细菌产生的那些，是最安全和最有效的昆虫控制剂之一。表达此类蛋白质的转基因 (GM) 作物是秋粘虫害虫综合防治 (IPM) 计划成功的关键部分。然而，由于人群对商业化转基因产品产生抗药性，迫切需要新的转基因特性。在此，我们描述了新设计的特征蛋白 eCry1Gb.1Ig 的进一步表征。与其他已充分表征的 Cry 蛋白类似，eCry1Gb.1Ig 显示可结合 FAW 中肠细胞并诱导细胞死亡。使用来自其他 FAW 活性事件的特征蛋白进行的结合竞争测定显示，当结合 FAW 刷状缘膜囊泡 (BBMV) 时以及在生物测定中使用非成孔版本作为竞争者时，缺乏竞争。同样，表达 2 和 3（现有商业 Cry 蛋白的已充分表征的受体）的昆虫细胞系对 eCry1Gb.1Ig 不敏感。这些发现与我们之前的工作结果一致，表明 eCry1Gb.1Ig 可有效控制对现有性状具有抗性的昆虫。这强调了 eCry1Gb.1Ig 作为一种具有独特作用位点的新型转基因特性蛋白的价值及其对全球粮食生产的潜在积极影响。