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Developmental validation of a custom‐designed Multi‐InDel panel: A five‐dye multiplex amplification system for challenging DNA samples
Electrophoresis ( IF 2.9 ) Pub Date : 2024-03-19 , DOI: 10.1002/elps.202470034
TABLE 1. Detailed information on primers, amplicon sizes, arrangement and the labeled fluorescence dyes for the 41 Multi-InDel markers.
更新日期：2024-03-19
Electrophoresis ( IF 2.9 ) Pub Date : 2024-03-19 , DOI: 10.1002/elps.202470034
This article corrects the following:
First published: 30 November 2023 | https://doi.org/10.1002/elps.202300192
In the article
Developmental validation of a custom-designed Multi-InDel panel: A five-dye multiplex amplification system for challenging DNA samples
By Qiong Lan, Meiming Cai, Hui Xu, Xiaole Bai, Chunmei Shen, and Bofeng Zhu; Electrophoresis 2024, 45, 489–504,
the authors would like to make the following corrections:
Table 1 in the original article contained errors and should be replaced by Table 1 below:
| Markers | RS | Positions | Ref Alleles | Alt Alleles | Primer sequences | Primer concentrations | Allele 1 | Allele 2 | Allele 3 | Allele 4 | Fluorescent dyes |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MI7 | rs145695868 | 143606981 | CAA | C | F: ACTCAGATTTGCTGTAGTAAAACTGT | 0.7408 | / | 81.48 | 83.37 | 85.35 | FAM |
| rs10662420 | 143606995 | T | TAGAG | R: CTAATCTGTCTTTTCTCTCTGGTTG | 0.7408 | ||||||
| MI18 | rs11435843 | 71926279 | C | CT | F: TCTTAACAGGAATTCCATTTTTAGTGCT | 0.6636 | 99.46 | / | 104.47 | 105.57 | |
| rs71300657 | 71926325 | G | GTTACT | R: AGCAAAAAGAAGTTATACTTGGCAT | 0.6636 | ||||||
| MI13 | rs3214372 | 12230939 | A | AT | F: GATGTTTTTCGAGTAAGAAGTCATGAATAT | 1.3273 | / | 110.95 | / | 113.76 | |
| rs3217132 | 12230957 | CTTA | C | R: CCTATCTTAAAGCTAACAGAGATGC | 1.3273 | ||||||
| 2MI32 | rs35211839 | 136043020 | TC | T | F: CCAATGTTTTATTTAGGTACCTGTAATGGGA | 0.8334 | 129.61 | / | 132.6 | 133.57 | |
| rs74678400 | 136043027 | T | TCTC | R: AGTCTGGGGAGAATTTAAACAGGAAAC | 0.8334 | ||||||
| MI6 | rs57913087 | 129588428 | GC | G | F: TGTTCCCAAGCCAGGACACT | 0.8334 | 140.89 | / | 145.03 | / | |
| rs34358108 | 129588471 | TGTGA | T | R: GGACAGGAAAAATGTGGGGCTCG | 0.8334 | ||||||
| MI17 | rs35838770 | 15547647 | TTGAG | T | F: GTCGTAGATTGAATGTTTATGTTCCCA | 0.9414 | / | 150 | 153.24 | 154.15 | |
| rs11434204 | 15547688 | A | AT | R: TGGGGTCCTATTTATAAGGGCACTAA | 0.9414 | ||||||
| MI4 | rs57803994 | 120353840 | TC | T | F: AATTCCTCAGCGACTCAGTGGAGC | 1.0958 | / | / | 168.01 | 168.93 | |
| rs149065177 | 120353875 | AGTACTT | A | R GGCTGGGAGAGAAAACACAGACA | 1.0958 | ||||||
| 2MI55 | rs11383217 | 3595790 | TG | T | F: TCCAGTAGGGAATGTGATCAGG | 0.8488 | 177.53 | / | 181.59 | 182.6 | |
| rs3072903 | 3595799 | TGGTA | T | R: GAAGTGTTCTGTCTGACTCAGCAGT | 0.8488 | ||||||
| MI1 | rs375064432 | 54821200 | A | ATTGT | F: AGTTTGCAAACATTTTCTCCTATTCTACA | 0.926 | 185.14 | 186.15 | / | 190.22 | |
| rs35924832 | 54821219 | G | GC | R: GACATAAGGGAAAAACTTTAGGACACTG | 0.926 | ||||||
| 2MI26 | rs137991189 | 19828048 | C | CAATT | F: AATTGATAAGGTGGTGAGATAATGGA | 1.0186 | / | 198.37 | 201.33 | 202.25 | |
| rs11375636 | 19828090 | TC | T | R: ATAACAATCCTTGAACAGCAAGTTCAGAG | 1.0186 | ||||||
| 2MI17 | rs35515881 | 115333796 | A | AC | F: TCATCTTACAAAGTATCCTAAACCCTGT | 0.8951 | / | 208.51 | 211.61 | 212.54 | |
| rs10545807 | 115333801 | A | ATTGT | R: GAAGAGTCCTAAGGGGGACTTTC | 0.8951 | ||||||
| 2MI36 | rs144820685 | 124264097 | C | CCTT | F: AGAAAATCAAAGGAAATACTGAGGTAGTAAG | 1.4816 | 217.44 | / | 220.47 | 221.42 | |
| rs67660578 | 124264145 | G | GT | R: GTAAAATAGCCTCTTTGTGTCATGACT | 1.4816 | ||||||
| MI12 | rs10542319 | 76450170 | TAAGTAA | T | F: GTAAATAACTGAAACCCACTTAAGAGCT | 1.2347 | / | 83.71 | 86.74 | 89.6 | JOE |
| rs201032359 | 76450186 | AATT | A | R: TCTGCTTTAGGCTTCCATGAGATAC | 1.2347 | ||||||
| 2MI06 | rs200948419 | 1197935 | T | TAGAC | F: AATGAAAATAATTGGGGATTTCAACACTG | 1.5433 | / | 98.39 | 101.45 | 102.46 | JOE |
| rs35727128 | 1197949 | AG | A | R: AGTCTAAAGTACAAGTTAAATCCAATGC | 1.5433 | ||||||
| MI40 | rs71823338 | 50725583 | C | CT | F: GTTTGTGATGTCTGAGTCCAGTCCTG | 2.778 | 106.25 | / | 109.27 | 110.3 | |
| rs149565141 | 50725585 | TGTG | T | R: AGAGAGTACCGGACTCCAGCCATTA | 2.778 | ||||||
| 2MI51 | rs141490808 | 33342609 | C | CTTGT | F: TAAGAATCATTTTCAAGGAAAATGGGT | 1.8057 | / | 119.43 | 122.59 | 123.65 | |
| rs147923862 | 33342611 | T | TG | R: GGGAAGGATTTTGAGGTAGAAAACT | 1.8057 | ||||||
| 2MI31 | rs113897607 | 126439578 | T | TATATC | F: GTGAATTTGGGCTACTACACAAAACT | 2.0063 | 130.47 | 134.64 | 135.61 | / | |
| rs34846650 | 126439607 | A | AT | R: TCTCAAATAACTTTTGGATGTTGAGATTATA | 2.0063 | ||||||
| MI30 | rs199632543 | 89276644 | G | GATA | F: ATACTATCGAATTTGAATTTCATGCAATTTTCAC | 1.6051 | 145.53 | 146.63 | 149.5 | / | |
| rs72512782 | 89276650 | A | AT | R: AGATTTATTGGCCAAATTCAGCCAATT | 1.6051 | ||||||
| 2MI49 | rs377114040 | 88300842 | A | AGTTTT | F: GATTGAGTCCAGAATTCCAAAATTCAGAAGG | 2.0063 | 155.87 | / | 160.86 | 162.9 | |
| rs3055901 | 88300854 | C | CAT | R: CCACTGGTCTATATTTGCTCAGATGAAGA | 2.0063 | ||||||
| 2MI25 | rs11404703 | 77671704 | AT | A | F: TTTATTAACTCATTGTAGCCCCTCATTATC | 1.2038 | 166.58 | / | 172.94 | 173.87 | |
| rs145084515 | 77671716 | A | AGTTGAC | R: GAATTGAACTGCCCTGTTCATTCCCC | 1.2038 | ||||||
| 2MI48 | rs5808095 | 40574532 | TAG | T | F: ATAAATTTCACCTTTAAAGTCTAGAATTATGC | 2.0063 | 177.94 | / | / | 183.82 | |
| rs5808096 | 40574547 | TATTA | T | R: AATGAGTGTATGTTAAACTGATAGATTAGATA | 2.0063 | ||||||
| MI26 | rs35126629 | 100126537 | CTCTG | C | F: ATTTAGGATAGCTAACTCTTCTTGTTGA | 1.8057 | / | 192.36 | / | 196.33 | |
| rs71465310 | 100126551 | TA | T | R: GGACATACATAGGCTGAAAGTAAAGGG | 1.8057 | ||||||
| 2MI18 | rs141416140 | 17025053 | C | CTTAT | F: AATTTGAAGAGGAGCACTCTCTTAAAC | 1.8057 | 210.68 | / | 213.58 | 214.62 | |
| rs56114052 | 17025085 | G | GT | R: GAAGAAAGTGTTTCTCCCAGTAAATTATCAT | 1.8057 | ||||||
| MI14 | rs71766343 | 30789162 | AGGACCGGT | A | F: CCCTAAAAGGATCTGGGGACT | 0.5402 | 89.08 | / | 97.15 | 99.29 | TAMRA |
| rs56207509 | 30789175 | CAG | C | R: GCATGCTAAGACAGGACCCTGGC | 0.5402 | ||||||
| 2MI58 | rs72004284 | 52344782 | A | ACTT | F: CTTTGGAGAATTTGCCATCCCAAAC | 0.4013 | 110.95 | / | 114.04 | 114.98 | |
| rs139944155 | 52344786 | CG | C | R: GAAGAAATACCCGGAAACAACTGCAT | 0.4013 | ||||||
| 2MI44 | rs59552010 | 39251896 | G | GAGACAA | F: ACAGCAAAAGAGCATCAATAAATAAG | 0.6636 | 121.44 | / | 127.3 | 129.22 | |
| rs61656423 | 39251900 | CTA | C | R: ATAGTTAATACTATCATTATTGCCACTCAA | 0.6636 | ||||||
| MI23 | rs3041403 | 53491025 | CCT | C | F: ATCTGCTTTTAAATTGGTAATATGAGTGAAA | 0.6482 | 134.93 | 136.98 | / | 141.19 | |
| rs143792664 | 53491074 | T | TAGTC | R: CACCATGTCTGGACAATTTTTGTTA | 0.6482 | ||||||
| MI32 | rs35880714 | 93447644 | A | AT | F: ACTATCTTGAAAGGAATGACAGATTTACAAT | 0.926 | 145.03 | / | 150.1 | 151.11 | |
| rs145177299 | 93447660 | CTTTTA | C | R: TGCGATGGGTTGATCTGTGCACACCATG | 0.926 | ||||||
| 2MI33 | rs35002191 | 8604012 | AT | A | F: ATAGAGCTGGAGAATATGGCAGCG | 0.8334 | 154.05 | / | 158.01 | 159.02 | |
| rs56939199 | 8604018 | G | GGTAT | R: ATCTATCTCATTAAAAGGATAGGTACTAAACA | 0.8334 | ||||||
| MI33 | rs4065037 | 40058303 | A | AGAG | F: GAAAGGCAGAATTTGAATCAACTCTTGG | 0.5402 | 162.8 | 165.76 | / | 173.15 | TAMRA |
| rs371735069 | 40058327 | G | GCCCAGAC | R: CCATACAAAGGAAATGGCAGGC | 0.5402 | ||||||
| MI5 | rs11455726 | 195674823 | T | TA | F: TATACACCTACCTATAGAGTATAGCCAGTT | 0.3704 | 183.92 | / | / | 188.69 | |
| rs139352836 | 195674865 | TTGTA | T | R: TCATATTAGTGCCCAACACTGGT | 0.3704 | ||||||
| 2MI54 | rs570616838 | 12894366 | TTG | T | F: TCATTTGATTGTGATTGGTAGTAATGAGA | 0.7717 | 195.11 | 197.14 | / | / | |
| rs148035090 | 12894388 | GTCTA | G | R: ATTTGCCAAATCAAGCCATAAAGC | 0.7717 | ||||||
| 2MI10 | rs149741414 | 20184808 | C | CTATACT | F: TGTTGTACATACAGTTAAACGTTGAGT | 0.6173 | 105.39 | / | 111.42 | 112.54 | ROX |
| rs58466926 | 20184837 | A | AG | R: AGTGGTTTGACATGTTTAGTAGC | 0.6173 | ||||||
| MI38 | rs150531146 | 59784328 | ATATG | A | F(1): AGCATGAGTCAATTCCTTAAAATAAATG | 0.4321 | / | 130.5 | 132.6 | / | |
| F(2): AGCATGAGTCAATTCCTTAAAATAAATT | 0.6482 | ||||||||||
| rs200084710 | 59784330 | ATG | A | R: ATACACATACATGTTTGTACATACACATTC | 1.0803 | ||||||
| MI24 | rs147938518 | 106288893 | ATTATG | A | F: GCTCAAAGAAAAAGTTTGCCTCAAAT | 0.5402 | / | / | 143.25 | 145.33 | |
| rs10550117 | 106288925 | TCA | T | R: TTTGATTGTCCTTATGTGATCTGGAAAC | 0.5402 | ||||||
| 2MI13 | rs144838892 | 3825465 | T | TTGA | F: TTTCTCTGACTCATTAATCTGGTTTCTA | 0.6173 | / | 152.12 | 154.35 | 155.27 | |
| rs200875353 | 3825486 | G | GC | R: ATTTGGCAAATATTTATTGTGTACCCAC | 0.6173 | ||||||
| 2MI07 | rs35291115 | 9899665 | T | TG | F: TGCAATTAGTGGATCATATAGTAAGTCTAT | 0.7717 | / | 160.65 | 164.63 | 165.76 | |
| rs77983960 | 9899689 | A | ATGTAT | R: GAGACTGTGATTTTCATCTCCTTCTTTGTA | 0.7717 | ||||||
| 2MI52 | rs3069843 | 94714153 | G | GATC | F: GTGTAAACTAAGATGGAGTACAAGCTGG | 0.4013 | 169.65 | / | 172.84 | 173.87 | |
| rs34063661 | 94714181 | CA | C | R: CAAAACAAGACAAAAACCTAGCCCTG | 0.4013 | ||||||
| 2MI16 | rs375709050 | 112703657 | T | TA | F: CTTTCCTACTTATAATATTGCATTGTATCTCTG | 1.034 | / | 179.66 | / | 183.72 | |
| rs79612828 | 112703667 | T | TTATC | R: AATCTAAATTTATGGTCTTCAATCTCTCC | 1.034 | ||||||
| 2MI15 | rs138642414 | 76137642 | CACTT | C | F: AGTAATAACAAATGGATTAACCATCAG | 0.8951 | 188.39 | 189.41 | / | / | |
| rs35703100 | 76137676 | A | AG | R: GCCTCGCTTTGTTGAAAGATGACATA | 0.8951 | ||||||
| 2MI19 | rs11286626 | 32848891 | T | TG | F: AGCCAATGTAAAATGAAGTTATGCTAG | 0.5556 | / | 205.42 | 208.51 | 209.44 | |
| rs3067346 | 32848928 | T | TTAAG | R: TGTGTGCATGTTACAACAGATTG | 0.5556 | ||||||
| Amelogenin | Amel | F(X): CCCTGGGCTCTGTAAAGAATAG | 0.3858 | / | 92(X) | 97.5(Y) | / | ||||
| Amel-X | F(Y): GCTGGTGGTAGGAACTGTAAAAT | 0.1286 | |||||||||
| Amel-Y | R(Universal): GCTGATGGTAGGAACTGTAAAAT | 0.2572 | |||||||||
- Note: The Multi-InDel markers are composed of two closely linked InDel loci in a short physical distance in the human genome, which could have four possible alleles (allele 1, allele 2, allele 3, and allele 4) in different populations. The detected alleles in the allelic ladder for this assay are shown in Table 1, with the undetected alleles labeled with “/”.
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