This work compares two bacterial isolates Streptomyces barkulensis RC1831 and Streptomyces chitinovorans RC1832 isolated from Chilika Lake sediments in Odisha, India, using whole-genome sequence analysis. According to the results of the genome analysis, the RC1831 genome has a chromosome with 6,383,258 bp (72.9% GC) and 6145 coding sequences and 66 RNA, while the RC1832 genome has a chromosome with 6,055,792 bp (73.1% GC) and 5824 coding sequences and 63 RNA. Further analysis of the carbohydrate active enzyme (CAZyme) revealed that RC1831 contains 78 glycoside hydrolase family genes, whereas RC1832 includes 50 glycoside hydrolases that have the potential to regulate the chitin-degrading enzymes. KAAS (KEGG Automatic Annotation Server) and AntiSMASH online tool V3.0.5 were used to identify a biosynthetic gene cluster in the isolated strain's genome. The detailed comparative analysis of the genes between the strains will help to gain better insight of chitin and other carbohydrate polymer degradation and secondary metabolite production in both the strains as well as the evolutionary relationship and possibilities of industrial application of these strains. Chitosan production might be explained by genes for the chitin breakdown pathway found in the genome sequence, but genes for later-stage conversion were not found. One significant biomolecule with a wide range of industrial uses is chitosan. Therefore, using these microbes to produce chitosan offers a viable waste disposal solution.

这项工作使用全基因组序列分析，比较了从印度奥里萨邦 Chilika 湖沉积物中分离出的两种细菌分离株Streptomyces barkulensis RC1831 和Streptomyces chitinovorans RC1832。根据基因组分析结果，RC1831基因组的染色体有6,383,258 bp（72.9% GC）、6145个编码序列和66个RNA，而RC1832基因组有6,055,792 bp（73.1% GC）的染色体和5824个编码序列和63个RNA。对碳水化合物活性酶（CAZyme）的进一步分析表明，RC1831包含78个糖苷水解酶家族基因，而RC1832包含50个糖苷水解酶，具有调节几丁质降解酶的潜力。使用KAAS（KEGG自动注释服务器）和AntiSMASH在线工具V3.0.5鉴定分离菌株基因组中的生物合成基因簇。对菌株之间基因的详细比较分析将有助于更好地了解两个菌株中几丁质和其他碳水化合物聚合物的降解和次生代谢产物的产生，以及这些菌株的进化关系和工业应用的可能性。壳聚糖的产生可能是通过基因组序列中发现的几丁质分解途径的基因来解释的，但没有发现后期转化的基因。壳聚糖是一种具有广泛工业用途的重要生物分子。因此，利用这些微生物生产壳聚糖提供了一种可行的废物处理解决方案。