Abstract

Human T-lymphotropic virus type 1 (HTLV-1) is associated with the development of malignant diseases, particularly adult T-cell leukemia/lymphoma (ATLL) and myelopathy/tropical spastic paraparesis (HAM/TSP). Hemophilia patients are at risk of acquiring blood–borne infections, making it critical to prevent the transmission of viral and other contaminants. The objective of this study was to determine the prevalence of HTLV-1 in Iranian hemophilia patients who were referred to the Iranian Comprehensive Hemophilia Care Center (ICHCC) and to analyze the results. A total of 320 blood samples were collected from hemophilia patients and screened using enzyme-linked immunosorbent assay (ELISA). Seropositive samples were confirmed by amplifying the long terminal repeat (LTR) region of HTLV-1 using nested PCR. The LTR region fragment was amplified, sequenced, and analyzed by MEGA 7 for phylogenetic analysis. Three out of four positive serological samples were confirmed using PCR, resulting in an HTLV-1 infection outbreak of 0.9%. Phylogenetic analysis of hemophilia patients infected with HTLV-1 revealed that the virus belongs to subtype a (Cosmopolitan) and subgroup A (Transcontinental). The findings suggest that hemophilia patients may be at high risk for HTLV-1 transmission. Furthermore, screening of blood and blood products can play a critical role in preventing the spread of the virus in endemic areas.

中文翻译：

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血友病患者 HTLV-1 病毒的系统发育分析和频率估计参考伊朗综合血友病护理中心 (ICHCC)

摘要

人类 T 淋巴细胞病毒 1 型 (HTLV-1) 与恶性疾病的发生有关，特别是成人 T 细胞白血病/淋巴瘤 (ATLL) 和脊髓病/热带痉挛性截瘫 (HAM/TSP)。血友病患者面临血液传播感染的风险，因此防止病毒和其他污染物的传播至关重要。本研究的目的是确定转诊至伊朗综合血友病护理中心 (ICHCC) 的伊朗血友病患者中 HTLV-1 的患病率并分析结果。总共从血友病患者采集了 320 份血液样本，并使用酶联免疫吸附测定 (ELISA) 进行筛查。通过使用巢式 PCR 扩增 HTLV-1 的长末端重复 (LTR) 区域来确认血清阳性样本。LTR 区域片段通过 MEGA 7 进行扩增、测序和分析，以进行系统发育分析。四分之三的阳性血清学样本经 PCR 确认，导致 HTLV-1 感染爆发率为 0.9%。对感染HTLV-1的血友病患者的系统发育分析显示，该病毒属于a亚型（Cosmopolitan）和A亚组（TransContinental）。研究结果表明，血友病患者可能面临 HTLV-1 传播的高风险。此外，血液和血液制品的筛查可以在防止病毒在流行地区传播方面发挥关键作用。