An inventory of parasites infecting the jaguar (Panthera onca) across its distribution range is relevant for the conservation of this threatened big cat. In this study, we report the occurrence of helminths in a jaguar from Mexico using morphological techniques (cleared and stained mounts and scanning electron microscopy) and partial sequences of the 28S ribosomal RNA (28S rRNA) gene and the cytochrome c oxidase 1 mitochondrial (COI) gene. We also provide an updated list of helminth species reported in jaguars in the Americas. Three helminth taxa are identified in the jaguar examined from Mexico: Toxocara cati, Physaloptera sp., and Taenia sp. The new 28S rRNA sequences of To. cati, Physaloptera sp., and Taenia sp. and the COI sequence of Taenia sp. corroborate the identity of the helminths isolated from this host. One hundred and twenty-nine records of helminths parasitizing jaguars from 49 studies up to May 2023 were identified in the Americas. In most of these studies (73.6%), helminths were identified using coproparasitological techniques. Sixteen helminths (7 nematodes, 5 cestodes, 3 acanthocephalans, and 1 trematode) were identified at the species level in free-ranging and captive jaguars. The study demonstrates the value of an integrative taxonomy approach to increase the accuracy of parasite identification in wildlife, especially when helminth specimens are scarce or poorly fixed.

美洲豹（Panthera onca）分布范围内感染寄生虫的清单与保护这种受威胁的大型猫科动物有关。在这项研究中，我们使用形态学技术（透明和染色的封片以及扫描电子显微镜）以及 28S 核糖体 RNA ( 28S rRNA) 基因和细胞色素 c 氧化酶 1 线粒体 ( COI ) 的部分序列，报告了墨西哥美洲虎体内蠕虫的发生情况。）基因。我们还提供了美洲虎身上报告的蠕虫物种的最新列表。在墨西哥检查的美洲虎中发现了三种蠕虫类群：猫弓蛔虫、轮纹虫和带绦虫。To的新28S rRNA 序列。cati、轮纹翅目 (Physaloptera sp.) 和绦虫属 ( Taenia sp.) 和带绦虫属的COI序列。证实从该宿主中分离出的蠕虫的身份。截至 2023 年 5 月，49 项研究在美洲发现了 129 条寄生虫记录。在大多数研究（73.6%）中，使用粪寄生虫学技术来鉴定蠕虫。在散养和圈养美洲虎的物种水平上鉴定出 16 种蠕虫（7 种线虫、5 种绦虫、3 种棘头虫和 1 种吸虫）。该研究证明了综合分类方法在提高野生动物寄生虫识别准确性方面的价值，特别是在蠕虫标本稀缺或固定不善的情况下。