The acaricidal effect of 14 strains of Metarhizium anisopliae sensu lato isolated from soil of livestock farms in the Mexican tropics was evaluated against larvae and engorged females, and during the laying and hatching of eggs of Rhipicephalus microplus (Ixodida: Ixodidae). For each fungal strain, the larvae mortality percentage was evaluated through a larval immersion test, while the reproductive efficiency indices in engorged females were measured using adult immersion tests at a dose of 1 × 10⁸ conidia/ml. All strains of M. anisopliae (s.l.) proved to be highly effective against R. microplus larvae (66–100%) and engorged females (100%). The strains also showed a good effect in inhibiting egg laying (16.45–56.38%) and a moderate effect in decreasing egg hatching (5.24–32.68%). Two strains demonstrated to be effective against all development phases of R. microplus in an integrated manner.

评估了从墨西哥热带畜牧场土壤中分离出的14 株绿僵菌（ Metarhizium anisopliae sensu lato）对幼虫和饱胀雌虫的杀螨效果，以及在小扇头扇贝（Ixodida：Ixodidae）的产卵和孵化过程中的杀螨效果。对于每种真菌菌株，通过幼虫浸泡试验评估幼虫死亡率，同时使用成虫浸泡试验以1×10 8^分生孢子/ml的剂量测量充血雌性的繁殖效率指数。所有M. anisopliae (sl)菌株均被证明对R. microplus幼虫（66-100%）和饱胀雌虫（100%）非常有效。该菌株还表现出良好的抑制产卵作用（16.45-56.38%）和中等程度的降低卵孵化作用（5.24-32.68%）。两种菌株被证明能够以综合方式有效对抗R. microplus的所有发育阶段。