Plants have very important chemical components, known as secondary metabolites, for the pharmaceutical industry, as well as for the chemical, cosmetics, and agricultural control industries. These secondary metabolites isolated from essential oils are used to obtain the raw material or fragrance component of the drug by semi-synthesis. For this reason, plants have been used to treat many diseases in the past, and their active ingredients are still used in medicine today. Each plant, each drug, contains differences owing to their natural structure. However, making the drug obtained from a plant a standard product is important in terms of using it as a medicine in treatment. Therefore, in our study, both the characterization of secondary metabolites and the antioxidant, antimicrobial and antiproliferative potential of Melissa officinalis were investigated. β-Citral (30.900%) was the main component of the essential oil. Total phenolic and flavonoid contents of M. officinalis were found to be 923.33 μg/mL gallic acid equivalent and 1.650 μg/mL quercetin equivalent. The free radical scavenging percentage of M. officinalis was 42.17%. M. officinalis had antimicrobial activity against Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Candida parapsilosis. In mouse fibroblast cells, the cell viability was found to be 87.50%, 88.235%, and 94.118% respectively, at low doses. In a human breast cancer cell line, it was observed that the cell viability at low concentrations was 77.861%, 85.40%, and 89.474% respectively. The inhibitory concentrations IC₅₀ of M. officinalis calculated for mouse fibroblast and human breast cancer cells in the GraphPad Prism 9.1.1 program were found to be 6229 and 4417 μg/mL respectively. In conclusion, M. officinalis has high bioactive secondary metabolites such as β-citral, β-caryophyllene, 6-methyl-5-hepten-2-one, and cis-1,2-dihydroperillaldehyde, has strong antimicrobial activity, and inhibits viability on breast cancer cells.

植物具有非常重要的化学成分，称为次生代谢物，对于制药工业以及化学、化妆品和农业控制工业而言。这些从精油中分离出来的次生代谢物用于半合成获得药物的原料或香料成分。因此，植物过去被用来治疗许多疾病，其活性成分至今仍用于医学。每种植物、每种药物因其天然结构而存在差异。然而，使从植物中获得的药物成为标准产品对于将其用作治疗药物而言很重要。因此，在我们的研究中，研究了香蜂草的次生代谢产物的表征以及抗氧化、抗菌和抗增殖潜力。β-柠檬醛（30.900%）是精油的主要成分。药用植物的总酚和黄酮含量为 923.33 μg/mL 没食子酸当量和 1.650 μg/mL 槲皮素当量。药用植物的自由基清除率为42.17%。药用植物对粪肠球菌、肺炎克雷伯菌、大肠杆菌、金黄色葡萄球菌和近平滑念珠菌具有抗菌活性。在小鼠成纤维细胞中，低剂量时细胞活力分别为 87.50%、88.235% 和 94.118%。在人乳腺癌细胞系中，观察到低浓度下的细胞活力分别为77.861%、85.40%和89.474%。在GraphPad Prism 9.1.1程序中计算得出的药用植物对小鼠成纤维细胞和人乳腺癌细胞的抑制浓度IC _{50分别为6229和4417 μg/mL。}综上所述，药用植物具有较高生物活性的次生代谢产物β-柠檬醛、β-石竹烯、6-甲基-5-庚烯-2-酮、顺式-1,2-二氢紫苏醛等，具有较强的抗菌活性，并具有抑制活力的作用。对乳腺癌细胞。