YARS is responsible for catalysing the binding of tyrosine to its cognate tRNA and plays a crucial role in basic biosynthesis. However, its biological functions in bladder cancer remains to be proven. We analysed variations in YARS1 expression and survival in bladder cancer using multiple data sets, including TCGA‐BLCA, GSE13507 and bladder cancer‐specific tissue microarrays. Furthermore, we explored the biological functions of YARS1 using transcriptome data. Our findings revealed a noteworthy correlation between YARS1 and immune infiltration in bladder cancer, as determined using the XCELL algorithm and single‐cell analysis. In addition, we employed the TIDE algorithm to evaluate the responsiveness of different cohorts to immune checkpoint therapy. We investigated the regulatory associations between YARS1 and various aspects of bladder cancer, including senescence, ferroptosis and stemness. Finally, we established a ceRNA network that is directly linked to the overall prognosis, YARS1 can serve as a prognostic biomarker for bladder cancer; its interaction with MYC has implications for bladder cancer cell senescence, ferroptosis and stemness. Moreover, the identified ceRNA network has potential as a therapeutic target in bladder cancer.

中文翻译：

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揭示 YARS1 在膀胱癌中的作用：预后生物标志物和治疗靶点

YARS 负责催化酪氨酸与其同源 tRNA 的结合，在基本生物合成中发挥着至关重要的作用。然而，其在膀胱癌中的生物学功能仍有待证实。我们使用多个数据集（包括 TCGA-BLCA、GSE13507 和膀胱癌特异性组织微阵列）分析了膀胱癌中 YARS1 表达和生存的变化。此外，我们利用转录组数据探索了 YARS1 的生物学功能。我们的研究结果揭示了 YARS1 与膀胱癌免疫浸润之间存在显着的相关性，这是使用 XCELL 算法和单细胞分析确定的。此外，我们采用 TIDE 算法来评估不同人群对免疫检查点治疗的反应性。我们研究了 YARS1 与膀胱癌各个方面之间的调节关联，包括衰老、铁死亡和干细胞性。最后，我们建立了一个与整体预后直接相关的ceRNA网络，YARS1可以作为膀胱癌的预后生物标志物；它与 MYC 的相互作用对膀胱癌细胞衰老、铁死亡和干性具有影响。此外，所鉴定的 ceRNA 网络有潜力作为膀胱癌的治疗靶点。