We have developed a highly sensitive and selective ferritin biosensor using the two dimensional (2D) fluorescent Magnesium Sulfide (MgS) nanosheets (NS) using ultra-sonication probe method. Ferritin (Fe) is spherical iron containing blood protein which acts as a storage house for iron. This Fe protein is an important biomarker for the detection of iron deficiency (anemia) such as, oxidative stress and cancer. Ultrasonication probe with one pot stirring method to synthesizethe 2D MgS NS was physicochemical, morphological, and analytical studied. The greenish-blue fluorescence of synthesized 2D MgS NS was optimized with maximum intensity at the excitation wavelength of 370 nm and emission wavelength of 435 nm. The quantum yield was 52.6 % found for fluorescent 2D MgS NS. The key role of 1-methylimidazole revealed 2D hexagonal nanosheets with high stability of fluorescence. Besides, the greenish-blue fluorescence of the proposed material was well quenched with the addition of Fe, resulting in a linear upward trend in the Stern-Volmer plot owing to static quenching. Further, the selectivity analysis of novel MgS NS shows an excellent selectivity for Fe in the presence of other analytes showing 77 % quenching efficiency and LOD 9 nM with a wide linear range of 10 to 200 nM for Fe detection. The proposed biosensor was successfully employed to detect Fe in blood serum samples with excellent linearity and applicability in clinical diagnosis.

中文翻译：

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用于荧光检测血清样品中铁蛋白的六角形二维硫化镁纳米片生物传感器：二维碱土金属硫化物（2D-AEMS）作为有效的荧光生物传感器

我们使用超声波探针方法使用二维（2D）荧光硫化镁（MgS）纳米片（NS）开发了一种高灵敏度和选择性的铁蛋白生物传感器。铁蛋白 (Fe) 是含有铁的球形血液蛋白，可作为铁的储存库。这种铁蛋白是检测氧化应激和癌症等缺铁（贫血）的重要生物标志物。对超声探针一锅搅拌法合成二维MgS NS进行了理化、形貌和分析研究。合成的 2D MgS NS 的绿蓝色荧光经过优化，在激发波长 370 nm 和发射波长 435 nm 处具有最大强度。荧光 2D MgS NS 的量子产率为 52.6%。 1-甲基咪唑的关键作用揭示了具有高荧光稳定性的二维六角形纳米片。此外，该材料的绿蓝色荧光随着Fe的添加而被很好地猝灭，由于静态猝灭，导致Stern-Volmer图呈现线性上升趋势。此外，新型 MgS NS 的选择性分析显示，在其他分析物存在的情况下，对 Fe 具有出色的选择性，显示出 77% 的淬灭效率和 LOD 9 nM，以及用于 Fe 检测的 10 至 200 nM 的宽线性范围。所提出的生物传感器已成功用于检测血清样品中的铁，具有良好的线性度和临床诊断的适用性。