This study aimed to investigate the effects of combined alpha-lipoic acid (ALA) and mitoquinone (Mito Q) supplementation on cardiac function and the underlying mechanisms in aged rats with myocardial infarction (MI). The aged rats underwent left anterior descending artery (LADA) occlusion for 30 min, followed by reperfusion for 24 h. ALA (100 mg/kg, gavage) and Mito Q (10 mg/kg, IP) were administered daily for two weeks before ischemia. Cardiac function, inflammatory, and apoptotic markers were evaluated 24 h after ischemia. The results of this study indicated that the administration of the combination of ALA and Mito Q significantly improved cardiac function. This improvement was linked to a reduction in the expression of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β ( < 0.001) and apoptotic markers (Bax, caspase-3, and Cyt-c), as well as a decrease in the percentage of TUNEL-positive cells (P < 0.001). The study revealed that combined intervention synergistically mitigated cardiac dysfunction by suppressing inflammatory and apoptotic pathways in aged rats with MI. Further research is needed to validate the potential of ALA and Mito Q as therapeutic options for elderly people at risk of heart attacks.

中文翻译：

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α-硫辛酸和米托醌联合补充对老年大鼠心肌梗死的影响：心脏性能和分子机制

本研究旨在探讨联合补充α-硫辛酸（ALA）和米托醌（Mito Q）对老年心肌梗死（MI）大鼠心脏功能的影响及其潜在机制。老年大鼠接受左前降支（LADA）闭塞30分钟，然后再灌注24小时。在缺血前两周，每天给予 ALA（100 mg/kg，灌胃）和 Mito Q（10 mg/kg，IP）。缺血24小时后评估心脏功能、炎症和细胞凋亡标志物。这项研究的结果表明，联合使用 ALA 和 Mito Q 可以显着改善心脏功能。这种改善与促炎细胞因子 TNF-α、IL-6 和 IL-1β ( < 0.001) 和凋亡标记物（Bax、caspase-3 和 Cyt-c）以及TUNEL 阳性细胞百分比减少 (P < 0.001)。研究表明，联合干预可通过抑制老年心肌梗死大鼠的炎症和细胞凋亡途径，协同减轻心脏功能障碍。需要进一步的研究来验证 ALA 和 Mito Q 作为有心脏病风险的老年人的治疗选择的潜力。