Photoelectrochemistry represents a promising technique for bioanalysis, though its application for the detection of Flap endonuclease 1 (FEN1) has not been tapped. Herein, this work reports the exploration of creating oxygen vacancies (Ov) onto the surface of BiOS nanosheets via the attachment of dopamine (DA), which underlies a new anodic PEC sensing strategy for FEN1 detection in label-free, immobilization-free and high-throughput modes. In connection to the target-mediated rolling circle amplification (RCA) reaction for modulating the release of the DA aptamer to capture DA, the detection system showed good performance toward FEN1 analysis with a linear detection range of 0.001–10 U/mL and a detection limit of 1.4 × 10 U/mL (S/N = 3). This work features the bioreaction engineered surface vacancy effect of BiOS nanosheets as a PEC sensing strategy, which allows a simple, easy to perform, sensitive and selective method for the detection of FEN1. This sensing strategy might have wide applications in versatile bioasssays, considering the diversity of a variety of biological reactions may produce the DA aptamer.

中文翻译：

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目标介导的生物反应设计 Bi2O2S 纳米片的表面空位效应，用于 FEN1 的光电化学检测

光电化学是一种很有前景的生物分析技术，但其在检测 Flap 核酸内切酶 1 (FEN1) 方面的应用尚未得到开发。在此，这项工作报告了通过附着多巴胺 (DA) 在 BIOS 纳米片表面创建氧空位 (Ov) 的探索，这是一种新的阳极 PEC 传感策略的基础，用于无标记、无固定化和高浓度的 FEN1 检测。 -吞吐量模式。与用于调节 DA 适体释放以捕获 DA 的靶介导滚环扩增 (RCA) 反应相关，检测系统对 FEN1 分析表现出良好的性能，线性检测范围为 0.001–10 U/mL，检测限值为 1.4 × 10 U/mL (S/N = 3)。这项工作以 BIOS 纳米片的生物反应工程表面空位效应作为 PEC 传感策略，为检测 FEN1 提供了一种简单、易于执行、灵敏和选择性的方法。考虑到多种生物反应可能产生 DA 适体的多样性，这种传感策略可能在多功能生物测定中具有广泛的应用。