The emergence of DNA nanotechnology has enabled the systematic design of diverse bionic dissipative behaviors under the precise control of nucleic acid nanodevices. Nevertheless, when compared to the dissipation observed in robust living systems, it is highly desirable to enhance the anti-interference for artificial DNA dissipation to withstand perturbations and facilitate repairs within the complex biological environments. In this study, we introduce strategically designed “trash cans” to facilitate kinetic control over interferences, transforming the stochastic binding of individual components within a homogeneous solution into a competitive binding process. This approach effectively eliminates incorrect binding and the accumulation of systemic interferences while ensuring a consistent pattern of energy fluctuation from response to silence. Remarkably, even in the presence of numerous interferences differing by only one base, we successfully achieve complete system reset through multiple cycles, effectively restoring the energy level to a minimum. The system was able to operate stably without any adverse effect under conditions of irregular interference, high-abundance interference, and even multiplex interferences including DNA and RNA crosstalk. This work not only provides an effective paradigm for constructing robust DNA dissipation systems but also greatly broadens the potential of DNA dissipation for applications in high-precision molecular recognition and complex biological reaction networks.

中文翻译：

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通过消除系统指定误差的积累，构建稳定、鲁棒的抗干扰DNA耗散系统

DNA纳米技术的出现使得在核酸纳米器件的精确控制下多种仿生耗散行为的系统设计成为可能。然而，与在稳健的生命系统中观察到的耗散相比，非常需要增强人工DNA耗散的抗干扰性，以承受复杂的生物环境中的扰动并促进修复。在这项研究中，我们引入了战略性设计的“垃圾桶”，以促进对干扰的动力学控制，将均质溶液中各个成分的随机结合转变为竞争性结合过程。这种方法有效地消除了不正确的结合和系统干扰的积累，同时确保从响应到沉默的能量波动模式一致。值得注意的是，即使存在仅一个碱基不同的众多干扰，我们也通过多个周期成功实现了完整的系统重置，有效地将能量水平恢复到最低水平。该系统在不规则干扰、高丰度干扰，甚至DNA、RNA串扰等多重干扰的情况下都能稳定运行，无任何不利影响。这项工作不仅为构建强大的DNA耗散系统提供了有效的范例，而且极大地拓宽了DNA耗散在高精度分子识别和复杂生物反应网络中的应用潜力。