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Sandwich-type electrochemical aptasensor based on hemin-graphite oxide as a signal label and rGO/MWCNTs/chitosan/carbon quantum dot modified electrode for sensitive detection of Acinetobacter baumannii bacteria
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2024-03-16 , DOI: 10.1016/j.aca.2024.342491
Rokhsareh Abedi , Jahan Bakhsh Raoof , Mojtaba Mohseni , Ayemeh Bagheri Hashkavayi

() is a pathogenic bacterium that causes severe infections and its rapid and reliable diagnosis is essential for effective control and treatment. In this study, we present an electrochemical aptasensor based on a signal amplification strategy for the detection of , the high specificity and affinity of the aptamer for the target make it favorable for signal amplification. This allows for a highly sensitive and selective detection of the target. The aptasensor is based on a carbon screen-printed electrode (CSPE) that has been modified with a nanocomposite consisting of multi-walled carbon nanotubes (MWCNTs), reduced graphene oxide (rGO), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS. Additionally, the self-assembled aptamers were immobilized on hemin-graphite oxide (H-GO) as a signal probe.

中文翻译:

基于血红素-石墨氧化物作为信号标记和rGO/MWCNTs/壳聚糖/碳量子点修饰电极的三明治型电化学适体传感器用于鲍曼不动杆菌的灵敏检测

()是一种引起严重感染的病原菌,其快速、可靠的诊断对于有效控制和治疗至关重要。在本研究中,我们提出了一种基于信号放大策略的电化学适体传感器,用于检测,适体对靶标的高特异性和亲和力使其有利于信号放大。这允许对目标进行高度灵敏和选择性的检测。该适体传感器基于碳丝网印刷电极(CSPE),该电极经过纳米复合材料的改性,该纳米复合材料由多壁碳纳米管(MWCNT)、还原氧化石墨烯(rGO)、壳聚糖(CS)和合成碳量子点组成(CQD) 来自 CS。此外,自组装的适体被固定在氯化血红素-氧化石墨(H-GO)上作为信号探针。
更新日期:2024-03-16
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