, a pathogenic necrotizing fungus, induces diseases in grapevines such as grey mold, pre- and post-harvest decay, and a reduction in fruit quality. Plant biologists focus on L. genes () due to their crucial role in the plant defense system against diverse stresses. The objective of this study was to conduct an integrative analysis of genes, investigating their responses to through an in silico method followed by an in vitro approach. The goal was to establish a connection between the outcomes of both approaches, potentially offering complementary insights. The in silico approach involved the characterization of 74 genes within the grapevine's genome. This encompassed the identification of subcellular localization, transmembrane helices, evolutionary rates, promoter analysis, protein-protein interactions, and expression patterns in response to infection followed by a quantitative real-time PCR (qRT-PCR). A noteworthy discovery from our analysis revealed that 10 different genes have been linked to tolerance. This finding has the potential to serve as a model for future breeding programs.

中文翻译：

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葡萄 NAC 基因对灰霉病反应的综合分析

是一种致病性坏死真菌，会引起葡萄藤疾病，如灰霉病、收获前和收获后腐烂以及果实品质下降。植物生物学家关注 L. 基因，因为它们在植物防御系统抵御多种胁迫中发挥着至关重要的作用。本研究的目的是对基因进行综合分析，通过计算机方法和体外方法研究它们的反应。目标是在两种方法的结果之间建立联系，从而有可能提供互补的见解。计算机方法涉及葡萄基因组内 74 个基因的表征。这包括鉴定亚细胞定位、跨膜螺旋、进化速率、启动子分析、蛋白质-蛋白质相互作用以及感染响应的表达模式，然后进行定量实时 PCR (qRT-PCR)。我们的分析中有一个值得注意的发现，即 10 个不同的基因与耐受性相关。这一发现有可能成为未来育种计划的模型。