Guava root-knot nematode, has emerged as a major threat in tropical and sub-tropical countries across the globe. These nematodes utilize their esophageal gland-secreted protein, Translationally Controlled Tumour Protein (MeTCTP) for successful parasitism on guava plants. Our current study was focused on identifying the biomolecules produced by nematicidal endophytic bacterial strains crucial for suppressing MeTCTP. Results of our study exhibited that among different isolates (GREB1) demonstrated superior nematicidal efficacy and biomolecules from this strain, such as 2,1,3-benzothiodiazole (−6.0 kcal/mol), 2 hexen-1-ol (−5.1 kcal/mol) and heptanoic acid (−4.5 kcal/mol), exhibited notable binding affinity to MeTCTP protein in docking studies. Molecular dynamics simulation studies underscored the stability, dynamics, and binding mechanisms of these biomolecules with MeTCTP. Furthermore, the results of the assay with identified biomolecules proved that 2,1,3 benzothiadiazole @ 200 ppm had 99% juvenile mortality. The probit analysis revealed the LC concentration of 2,1,3 benzothiadiazole (52.17 μg/mL), 2 hexen-1- ol (84.37 μg/mL) and heptanoic acid (141.93 μg/mL) respectively. Integrating and bio-efficacy assays, our study provides a pivotal foundation for novel biomolecules against guava nematodes. Moreover, the study lays the foundation to formulate a novel solution comprising potent biomolecules to curb populations, offering a promising avenue for effective crop protection.

中文翻译：

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利用番石榴内微生物群中的杀线虫化合物对抗根结线虫、Meloidogyneenterolobii

番石榴根结线虫已成为全球热带和亚热带国家的主要威胁。这些线虫利用食管腺分泌的蛋白质——翻译控制肿瘤蛋白（MeTCTP）成功寄生在番石榴植物上。我们目前的研究重点是鉴定对抑制 MeTCTP 至关重要的杀线虫内生细菌菌株产生的生物分子。我们的研究结果表明，在不同的分离株（GREB1）中，该菌株表现出优异的杀线虫功效和生物分子，例如2,1,3-苯并硫二唑（−6.0 kcal/mol）、2 hexen-1-ol（−5.1 kcal/ mol）和庚酸（-4.5 kcal/mol）在对接研究中表现出与 MeTCTP 蛋白显着的结合亲和力。分子动力学模拟研究强调了这些生物分子与 MeTCTP 的稳定性、动力学和结合机制。此外，已鉴定生物分子的测定结果证明，200 ppm 的 2,1,3 苯并噻二唑的幼年死亡率为 99%。概率分析显示，LC 浓度分别为 2,1,3 苯并噻二唑 (52.17 μg/mL)、2-己烯-1-醇 (84.37 μg/mL) 和庚酸 (141.93 μg/mL)。整合和生物功效测定，我们的研究为抗番石榴线虫的新型生物分子提供了关键基础。此外，该研究为制定一种包含有效生物分子的新型解决方案来抑制种群奠定了基础，为有效的作物保护提供了一条有希望的途径。