The galactomannan-based gel from seeds was used to incorporate sp. UCP 1286 and commercial collagenases. Experiments were carried out according to a 2-full factorial design to identify the most significant parameters for the incorporation process. The pH of the incorporation solution (pH), stirring time (), and initial protein concentration in the crude extract () were selected as the three independent variables, and the efficiency of collagenase incorporation () and collagenolytic activity () after 360 min as the responses. pH and showed positive statistically significant effects on , while was positively influenced by pH and , but negatively by . The fungi collagenase was released from the gel following a pseudo-Fickian behavior. Additionally, no <76 % of collagenase was efficiently incorporated into the gel retaining a high CA (32.5–69.8 U/mL). The obtained results for the commercial collagenase ( = 93.88 %, CA = 65.8 U/mL, and = 0.10) demonstrated a pseudo-Fickian behavior similar to the fungi-collagenase. The results confirm the biotechnological potential of the gel as an efficient matrix for the incorporation of catalytic compounds; additionally, the incorporation of collagenases was achieved by retaining the proteases CA and releasing them in a controlled manner.

中文翻译：

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决明子半乳甘露聚糖凝胶在生物医学用途中的应用：胶原酶的掺入及其释放曲线的研究

来自种子的基于半乳甘露聚糖的凝胶用于掺入 sp。 UCP 1286 和商业胶原酶。实验根据2全因子设计进行，以确定掺入过程中最重要的参数。选择掺入溶液的pH值（pH）、搅拌时间（）和粗提物中的初始蛋白浓度（）作为三个自变量，将胶原酶掺入效率（）和360分钟后的胶原蛋白分解活性（）表示为的回应。 pH 和 对 呈正向统计显着影响，而 pH 和 呈正向影响，但呈负向影响。真菌胶原酶按照伪菲克行为从凝胶中释放。此外，没有<76%的胶原酶被有效地掺入凝胶中，保留了高CA (32.5–69.8 U/mL)。商业胶原酶的获得结果（= 93.88 %，CA = 65.8 U/mL，并且 = 0.10）证明了与真菌胶原酶类似的伪 Fickian 行为。结果证实了凝胶作为掺入催化化合物的有效基质的生物技术潜力；此外，胶原酶的掺入是通过保留蛋白酶CA并以受控方式释放它们来实现的。