Diabetic wound infection brings chronic pain to patients and the therapy remains a crucial challenge owing to the disruption of the internal microenvironment. Herein, we report a nano-composite hydrogel (ZnO@HN) based on ZnO nanoparticles and a photo-trigging hyaluronic acid which is modified by o-nitrobenzene (NB), to accelerate infected diabetic wound healing. The diameter of the prepared ZnO nanoparticle is about 50 nm. X-ray photoelectron spectroscopy (XPS) analysis reveals that the coordinate bond binds ZnO in the hydrogel, rather than simple physical restraint. ZnO@HN possesses efficient antioxidant capacity and it can scavenge DPPH about 40 % in 2 h and inhibit HO >50 % in 8 h. The nano-composite hydrogel also exhibits satisfactory antibacterial capacity (58.35 % against and 64.03 % against for 6 h). tests suggest that ZnO@HN is biocompatible and promotes cell proliferation. experiments reveal that the hydrogel can accelerate the formation of new blood vessels and hair follicles. Histological analysis exhibits decreased macrophages, increased myofibroblasts, downregulated TNF-α expression, and enhanced VEGFA expression during wound healing. In conclusion, ZnO@HN could be a promising candidate for treating intractable infected diabetic skin defection.

中文翻译：

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纳米复合透明质酸水凝胶可有效抗菌并清除ROS，促进感染的糖尿病伤口愈合

糖尿病伤口感染给患者带来慢性疼痛，由于内部微环境的破坏，治疗仍然是一个严峻的挑战。在此，我们报道了一种基于 ZnO 纳米粒子和经邻硝基苯 (NB) 修饰的光触发透明质酸的纳米复合水凝胶 (ZnO@HN)，可加速感染的糖尿病伤口愈合。所制备的ZnO纳米颗粒的直径约为50 nm。 X射线光电子能谱（XPS）分析表明，配位键结合水凝胶中的ZnO，而不是简单的物理约束。 ZnO@HN具有高效的抗氧化能力，2小时内可清除约40%的DPPH，8小时内抑制H2O>50%。纳米复合水凝胶还表现出令人满意的抗菌能力（6小时抗菌能力为58.35%和64.03%）。测试表明ZnO@HN具有生物相容性并促进细胞增殖。实验表明，水凝胶可以加速新血管和毛囊的形成。组织学分析显示伤口愈合过程中巨噬细胞减少、肌成纤维细胞增加、TNF-α 表达下调、VEGFA 表达增强。总之，ZnO@HN 可能是治疗顽固性感染性糖尿病皮肤缺损的有希望的候选者。