Sunset Yellow, a synthetic orange azo food dye was examined in this study for its impact on the Wistar rat brain sub-regions. The dye was administered orally to weanling rats at the Acceptable Daily Intake level (4 mg/kg/bw) for 40 days, and brain sub-regions viz., frontal cortex, cerebellum and hippocampus were examined for biochemical and histopathological changes. The results showed a significant decrease in tissue protein levels, superoxide dismutase, and catalase activity, as well as a significant increase in lipid peroxide levels in all brain sub-regions. Glutathione-S-transferase and Glutathione Reductase activities decreased, while Glutathione peroxidase activity increased. The biogenic amine levels and Acetylcholinesterase activity were also altered, with the frontal cortex and hippocampus being the most affected. Additionally, the dye caused histopathological damage in all brain sub-regions examined. This study indicates that the ADI level of Sunset Yellow may adversely affect brain tissue by causing oxidative damage.

中文翻译：

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日落黄诱导大鼠大脑亚区域的生化和组织病理学改变

本研究检查了日落黄（一种合成的橙色偶氮食用染料）对 Wistar 大鼠大脑亚区域的影响。以可接受的每日摄入量（4 mg/kg/bw）向断奶大鼠口服染料，持续 40 天，并检查大脑亚区域（即额叶皮层、小脑和海马）的生化和组织病理学变化。结果显示，所有大脑亚区域的组织蛋白水平、超氧化物歧化酶和过氧化氢酶活性显着降低，并且脂质过氧化物水平显着增加。谷胱甘肽-S-转移酶和谷胱甘肽还原酶活性下降，而谷胱甘肽过氧化物酶活性增加。生物胺水平和乙酰胆碱酯酶活性也发生改变，其中额叶皮层和海马体受影响最大。此外，该染料对所有检查的大脑亚区域造成组织病理学损伤。这项研究表明，日落黄的 ADI 水平可能会导致氧化损伤，从而对脑组织产生不利影响。