We compared transamniotic stem cell therapy (TRASCET) using either mesenchymal (MSCs) or hematopoietic (HSCs) stem cells on fetal hematopoiesis in a syngeneic model of intrauterine growth restriction (IUGR). Lewis dams exposed to cycling hypoxia (10.5% O) in late gestation had their fetuses (n = 83) either receiving no intervention (untreated; n = 9), or intra-amniotic injections of either HSCs (HSC; n = 34), MSCs primed to an enhanced anti-inflammatory phenotype (primed-MSC; n = 28), or saline (sham; n = 12). Normal controls (n = 18) were also studied. Complete peripheral blood counts and placental ELISA for inflammation and angiogenesis markers were performed at term. Overall survival from hypoxia was 41% (34/83). Red blood count (RBC), hematocrit (Hct) and hemoglobin levels (Hb) were all significantly decreased from normal in all hypoxia groups. TRASCET with primed-MSC had significantly higher RBC, Hct, and Hb levels than sham (p = 0.01–0.03, pairwise), though not than untreated (which had no surgical blood loss). The HSC group had only significantly higher Hb levels than sham (p = 0.005). TRASCET with primed-MSC had significantly lower levels of placental TNF-α than sham (p = 0.04), but not untreated. MCSs seem more effective than HSCs in enhancing hematopoiesis when used as donor cells for TRASCET in a syngeneic model of IUGR. N/A (animal and laboratory study).

中文翻译：

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在子宫内生长受限的同基因模型中，间充质干细胞和造血干细胞作为经羊膜干细胞疗法 (TRASCET) 对胎儿造血和胎盘炎症的比较影响

我们在宫内生长受限（IUGR）同基因模型中比较了使用间充质（MSC）或造血（HSC）干细胞的经羊膜干细胞疗法（TRASCET）对胎儿造血的影响。在妊娠后期暴露于循环缺氧 (10.5% O) 的 Lewis 母鼠的胎儿 (n = 83) 要么不接受干预（未经治疗；n = 9），要么羊膜内注射 HSC (HSC；n = 34)，已引发增强抗炎表型的 MSC（已引发的 MSC；n = 28）或生理盐水（假手术；n = 12）。还对正常对照（n = 18）进行了研究。足月时进行完整的外周血计数和胎盘 ELISA 检测炎症和血管生成标记物。缺氧的总生存率为 41% (34/83)。所有缺氧组的红细胞计数（RBC）、血细胞比容（Hct）和血红蛋白水平（Hb）均较正常值显着下降。与假手术相比，经过预处理的 MSC 的 TRASCET 的 RBC、Hct 和 Hb 水平显着高于假手术（p = 0.01-0.03，成对），但并不比未治疗（没有手术失血）高。 HSC 组的 Hb 水平仅显着高于假手术组 (p = 0.005)。与假手术相比，经过预处理的 MSC 的 TRASCET 胎盘 TNF-α 水平显着降低 (p = 0.04)，但并非未经治疗。当在 IUGR 同基因模型中用作 TRASCET 的供体细胞时，MCS 在增强造血方面似乎比 HSC 更有效。 N/A（动物和实验室研究）。