We have recently characterized experimental autoimmune encephalomyelitis (EAE) induced in DA rats with spinal cord homogenate without complete Freund's adjuvant (CFA). The main advantage of this multiple sclerosis model is the lack of CFA-related confounding effects which represent the major obstacles in translating findings from EAE to multiple sclerosis. Here, antigen specificity of the cellular and humoral immune response directed against the central nervous system was explored. The reactivity of and cells to myelin basic protein, myelin oligodendrocyte glycoprotein, and β-synuclein was detected. Having in mind that reactivity against β-synuclein was previously associated with autoimmunity against the brain, the infiltration of immune cells into different brain compartments, i.e. pons, cerebellum, hippocampus, and cortex was determined. cell infiltration was observed in all structures examined. This finding stimulated investigation of the effects of immunization on DA rat behavior using the elevated plus maze and the open field test. Rats recovered from EAE displayed increased anxiety-like behavior. These data support CFA-free EAE in DA rats as a useful model for multiple sclerosis research.

中文翻译：

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脊髓匀浆诱导暗刺豚鼠实验性自身免疫性脑脊髓炎的脑部炎症

我们最近对在不使用完全弗氏佐剂 (CFA) 的情况下使用脊髓匀浆在 DA 大鼠中诱导的实验性自身免疫性脑脊髓炎 (EAE) 进行了表征。这种多发性硬化症模型的主要优点是缺乏 CFA 相关的混杂效应，而这些混杂效应是将 EAE 的发现转化为多发性硬化症的主要障碍。在这里，探讨了针对中枢神经系统的细胞和体液免疫反应的抗原特异性。检测细胞对髓磷脂碱性蛋白、髓磷脂少突胶质细胞糖蛋白和β-突触核蛋白的反应性。考虑到针对β-突触核蛋白的反应性先前与针对大脑的自身免疫有关，因此确定了免疫细胞向不同脑室（即脑桥、小脑、海马和皮质）的浸润。在所有检查的结构中均观察到细胞浸润。这一发现激发了使用高架十字迷宫和旷场测试来研究免疫对 DA 大鼠行为的影响。从 EAE 中恢复的大鼠表现出更多的焦虑样行为。这些数据支持 DA 大鼠中不含 CFA 的 EAE 作为多发性硬化症研究的有用模型。