Tyrosinase (TYR) is a copper-containing oxidase that affects the synthesis of melanin in the human body, which is regulate to the pigmentation of the skin. Nevertheless, abnormal expression of TYR can lead to albinism, vitiligo and other skin diseases. Excessive accumulation of TYR is a marker of melanoma cancer and an important factor leading to pigmentation during wound healing, freckles and browning of fruits and vegetables. Efficient tracking of TYR is of significance for studying its pathophysiological mechanism. Herein, we synthesized a benzindole-based fluorescent probe - to detect TYR in living cells and zebrafish. The probe displayed a high selectivity and sensitivity in distinguishing TYR from other analytes with the low detection limit of 1.024 U/mL. Importantly, - was successfully used to imagine TYR at the wound site of broken tail of zebrafish.

中文翻译：

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用于斑马鱼断尾伤口部位酪氨酸酶成像的开启荧光探针

酪氨酸酶（TYR）是一种含铜氧化酶，影响人体内黑色素的合成，调节皮肤色素沉着。然而，TYR的异常表达可导致白化病、白癜风等皮肤病。 TYR的过量积累是黑色素瘤的标志，也是导致伤口愈合过程中色素沉着、雀斑以及水果和蔬菜褐变的重要因素。 TYR的有效追踪对于研究其病理生理机制具有重要意义。在此，我们合成了一种基于苯并吲哚的荧光探针 - 用于检测活细胞和斑马鱼中的 TYR。该探针在区分 TYR 和其他分析物方面表现出高选择性和灵敏度，检测限低至 1.024 U/mL。重要的是， - 成功地用于想象斑马鱼断尾伤口处的 TYR。