We appreciate Dr. Astrada’s interest in our recent article (1). Although he raised concern around using BMI as a screening parameter for our study, BMI was not the only “defining parameter” screening criteria in our study. Fasting glucose > 110mg/dL was also a critical exclusion criterion for the study which limits variation in metabolic characteristics in our study cohort. These and other criteria for participation in the study were reported in the original manuscript (2) from which the samples in our current study (1) were obtained. Furthermore, after participants were screened and included in the study, DEXA scans provide information on body composition, which we reported in the online supplemental material. Within a given age group, we found no differences in body composition (Fat %) between treatment groups at baseline. As for the participants’ daily activities, we did not collect occupation information, but an exclusion criteria for the study was participation in regular exercise (>20 minutes more than twice per week), which was reported in the original manuscript (2). Thus, all participants were not regular exercisers, further limiting baseline metabolic variability.

中文翻译：

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对评论信的回复：肌肉代谢组学和运动对心脏代谢风险的影响

我们感谢 Astrada 博士对我们最近的文章 (1) 的兴趣。尽管他对使用 BMI 作为我们研究的筛选参数表示担忧，但 BMI 并不是我们研究中唯一的“定义参数”筛选标准。空腹血糖> 110mg/dL 也是该研究的一个关键排除标准，它限制了我们研究队列中代谢特征的变化。这些和其他参与研究的标准已在原始手稿 (2) 中报告，我们当前研究 (1) 中的样本就是从原始手稿 (2) 中获得的。此外，在对参与者进行筛选并纳入研究后，DEXA 扫描可提供有关身体成分的信息，我们在在线补充材料中报告了这些信息。在给定的年龄组内，我们发现治疗组之间的身体成分（脂肪百分比）在基线时没有差异。至于参与者的日常活动，我们没有收集职业信息，但研究的排除标准是参加定期锻炼（每周两次以上> 20分钟），这在原稿（2）中有所报道。因此，所有参与者都不是定期锻炼者，进一步限制了基线代谢变异性。