ObjectiveTo explore the levels of expression and clinical role of peroxiredoxin 6 ( PRDX6) in lung adenocarcinoma.MethodsThis retrospective study used a series of bioinformatics methods to detect the levels of expression of and mutations in the PRDX6 gene in a range of cancers and lung adenocarcinoma. Immunohistochemistry was used to verify the levels of expression of PRDX6 protein in samples of lung adenocarcinoma compared with normal adjacent tissue. The effect of PRDX6 gene knockdown on the in vitro proliferation of a lung adenocarcinoma cell line was measured. Bioinformatics methods were used to determine the diagnostic value and impact on survival of the PRDX6 gene in patients with lung adenocarcinoma.ResultsThe results showed that the PRDX6 gene was highly expressed in lung adenocarcinoma and there were five mutations at different sites on the gene. PRDX6 promoted the proliferation of the lung adenocarcinoma cell line. The survival duration of lung adenocarcinoma patients with high levels of PRDX6 gene expression was significantly shorter than that of patients with low PRDX6 gene expression.ConclusionPRDX6 is highly expressed in lung adenocarcinoma and higher levels of expression of the PRDX6 gene were associated with a poorer prognosis.

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PRDX6在肺腺癌中的表达及临床作用

目的探讨过氧化物酶6（PRDX6）在肺腺癌中的表达水平及其临床作用。方法本回顾性研究采用一系列生物信息学方法检测PRDX6基因在多种癌症和肺腺癌中的表达水平和突变情况。采用免疫组织化学方法验证肺腺癌样本与正常癌旁组织相比PRDX6蛋白的表达水平。测量了 PRDX6 基因敲低对肺腺癌细胞系体外增殖的影响。采用生物信息学方法确定PRDX6基因对肺腺癌患者的诊断价值及对生存的影响。结果结果显示PRDX6基因在肺腺癌中高表达，且该基因不同位点存在5个突变。 PRDX6促进肺腺癌细胞系的增殖。 PRDX6基因高表达的肺腺癌患者生存期显着短于PRDX6基因低表达的患者。结论PRDX6在肺腺癌中高表达，PRDX6基因高表达与较差的预后相关。