Abstract

Operation of radio-genetic ‘Inherited sterility technique’ (IS), proposed for suppression of a serious tropical pest, Spodoptera litura (Fabr.) requires reproductively competitive moths as a prerequisite. Insects harbor microbes, which might be associated with their reproductive viability. The present study was attempted to assess the gut bacterial diversity of the F1 sixth instar (L6) progeny derived from irradiated (100Gy, 130Gy), sub-sterilized male parent moths in comparison with control, using Next Generation Sequencing (NGS) analysis of 16 S rRNA of the bacterial community of larval gut. Irradiated S.litura showed an increased bacterial load than control. Irradiation could stimulate the abundance of some groups of bacteria in the gut, whereas overall species richness and evenness were affected by irradiation. Phylum Firmicutes was the most abundant phylum in the irradiated larvae (both in the 100Gy F1L6 and 130Gy F1L6); whereas in control larval gut, the Proteobacteria, Bacteroidetes, Chlorofexi, and Cyanobacteria were found to be abundant as compared to the irradiated regimens. Lactobacillus, Bacillus, Paenibacillus, the genera belonging to the phylum Firmicutes were relatively more abundant in the irradiated larvae (100Gy F1L6 and 130Gy F1L6), whereas genera Pseudomonas, Neisseria, and Stenotrophomonas belonging to the phylum Proteobacteria were more abundant in control. The functional pathways involved in the oxidation and reduction of sulfate and nitrate were found to be enriched in the control larval gut whereas the streptomycin production, chloramphenicol degradation pathways were enriched in the irradiated larval gut. Irradiation could significantly alter the structure of S. litura bacteriome, which might be correlated with the degree of inherited sterility in this nuclear tactic, IS.

中文翻译：

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电离辐射介导来自受辐射父本的害虫斜纹夜蛾（夜蛾科：鳞翅目）幼虫肠道细菌群落组成的变化

摘要

为抑制严重的热带害虫斜纹夜蛾（Fabr. ）而提出的放射遗传“遗传不育技术”（IS）的操作需要具有繁殖竞争性的飞蛾作为先决条件。昆虫体内含有微生物，这可能与其繁殖能力有关。本研究试图使用下一代测序 (NGS) 分析 16 种蛾子，与对照相比，评估源自辐射（100Gy、130Gy）、亚灭菌父本蛾的 F1 第六龄 (L6) 后代的肠道细菌多样性。幼虫肠道细菌群落的 S rRNA。经辐照的斜纹夜蛾显示出比对照增加的细菌负荷。辐射可以刺激肠道中某些细菌群的丰度，而总体物种丰富度和均匀度则受到辐射的影响。厚壁菌门是受辐射幼虫中最丰富的门（100Gy F1L6 和 130Gy F1L6 均如此）；而在对照幼虫肠道中，与辐照方案相比，变形菌门、拟杆菌门、绿藻门和蓝藻门被发现丰富。辐照幼虫（100Gy F1L6 和 130Gy F1L6）中属于厚壁菌门的乳杆菌属、芽孢杆菌属、类芽孢杆菌属相对较多，而对照中属于变形菌门的假单胞菌属、奈瑟菌属和寡养单胞菌属较多。发现参与硫酸盐和硝酸盐氧化和还原的功能途径在对照幼虫肠道中富集，而链霉素产生、氯霉素降解途径在辐照幼虫肠道中富集。辐射可以显着改变斜纹夜蛾细菌组的结构，这可能与这种核策略 IS 的遗传不育程度相关。