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Efficacy of Cupressus sempervirens essential oils against Trogoderma granarium everts (Coleoptera: Dermestidae)
International Journal of Tropical Insect Science ( IF 1.2 ) Pub Date : 2024-03-21 , DOI: 10.1007/s42690-024-01204-w
Merzougui Abdelkader , Samir Tine , Fouzia Tine-Djebbar , Noureddine Soltani

The seeds and leaves of Cupressus sempervirens essential oils were evaluated against the khapra beetle, Trogoderma granarium at different concentrations. Mortality, repellence activity, adult emergence rate, larvae chemical profile (protein contents and energy reserves), seed germination effect, and grain damage were assessed. The results of phytochemical screening of C. sempervirens leaf and seed demonstrated the presence of saponins, flavonoids, leucoanthocyanins, catechetical tannins, and gallic tannins. We identified eighty-two (82) compounds for leaves and seventy-four (74) for seeds by GC/MS. The major compounds for leaf and seed EOs were α-pinene (33.65% and 26.53%), δ-3-Carene (14.78% and 19.48%) and terpinolene (4.25% and 4.13%), respectively. C. sempervirens exhibited fumigant toxicity and repellent activity as a function the concentrations. C. sempervirens leaves was most potent both in adult emergence (66.94%) and germination rate (99%) compared to seeds oil. Furthermore, the seed and leaf oils significantly reduced seed damage. Finally, the treatments induced an alteration of the biochemical profile via a decrease in energy reserves and protein content. The data obtained suggest that the toxicity of EO by fumigation could be explained by an alteration of insect metabolism via food uptake and digestion.



中文翻译:

柏木精油对谷斑皮蠹 (Trogoderma granarium) 外翻的功效(鞘翅目:皮科)

评估了不同浓度的柏木种子和叶子对抗卡普拉甲虫( Trogoderma granarium)的效果。评估死亡率、驱避活性、成虫羽化率、幼虫化学特征(蛋白质含量和能量储备)、种子发芽效果和籽粒损伤。C. sempervirens叶和种子的植物化学筛选结果表明存在皂苷、类黄酮、无色花青素、儿茶素单宁和没食子单宁。我们通过 GC/MS 鉴定了叶子中的八十二 (82) 种化合物和种子中的七十四 (74) 种化合物。叶子和种子EO的主要化合物分别是α-蒎烯(33.65%和26.53%)、δ-3-蒈烯(14.78%和19.48%)和萜品油烯(4.25%和4.13%)。C. sempervirens随浓度变化表现出熏蒸毒性和驱避活性。与种子油相比, C. sempervirens叶子的成虫出苗率 (66.94%) 和发芽率 (99%) 均最强。此外,种子油和叶油显着减少了种子损伤。最后,治疗通过能量储备和蛋白质含量的减少引起生化特征的改变。获得的数据表明,熏蒸造成的环氧乙烷毒性可以通过昆虫通过食物摄取和消化改变新陈代谢来解释。

更新日期:2024-03-21
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