Carbon dots (CDs) are a kind of novel carbon nanomaterial that has unique properties, which make them useful for various applications, and now newer types of CDs are being found. Herein, a series of easy, cost-effective, environmentally friendly and highly biocompatible one-step syntheses of fluorescent CDs were fabricated by reacting easily available aquatic waste used as the carbon source, using hydrothermal reaction. The rapid development of nanomaterials has provided unprecedented opportunities for various applications, and CDs have been widely used in pharmaceutical analysis, environmental monitoring, clinical diagnosis, medical imaging technologies and cancer treatment. Good biocompatibility is a prerequisite for the application of nanomaterials in biology, chemistry, medicine and other fields. This paper presents a systematic study of the biocompatibility and biosafety issues of the three as-synthesized CDs, including primary investigations of their cytotoxicity, cell morphology, plant imaging, apoptosis analysis and effects on embryos in zebrafish embryos. The aim of this research is to make a profound study on the biocompatibility of carbon dots and explore the application of CDs in the field of biological imaging.

碳点（CD）是一种新型碳纳米材料，具有独特的性能，使其可用于各种应用，现在正在发现新型的碳点。在此，通过水热反应将容易获得的水生废物用作碳源，制备了一系列简单、经济有效、环境友好且高度生物相容性的荧光CD的一步合成。纳米材料的快速发展为各种应用提供了前所未有的机遇，CD已广泛应用于药物分析、环境监测、临床诊断、医学成像技术和癌症治疗等领域。良好的生物相容性是纳米材料在生物、化学、医学等领域应用的前提。本文对三种合成CD的生物相容性和生物安全性问题进行了系统研究，包括其细胞毒性、细胞形态、植物成像、细胞凋亡分析以及对斑马鱼胚胎的影响的初步研究。本研究旨在深入研究碳点的生物相容性，探索碳点在生物成像领域的应用。