Recent studies have demonstrated that APOC1 is associated with cancer progression, exerting cancer-promoting and immune infiltration-promoting effects. Nevertheless, there is currently no report on the presence of APOC1 in ovarian cancer (OV). In this study, we conducted data analysis using the GEO and TCGA databases. We conducted a thorough bioinformatics analysis to investigate the function of APOC1 in OV, utilizing various platforms including cBioPortal, STRING, GeneMANIA, LinkedOmics, GSCALite, TIMER, and CellMarker. Additionally, we performed immunohistochemical staining on tissue microarrays and conducted in vitro cellular assays to validate our findings. Our findings reveal that APOC1 expression is significantly upregulated in OV compared to normal tissues. Importantly, patients with high APOC1 levels show a significantly poorer prognosis. Furthermore, our study demonstrated that APOC1 exerted a crucial function in promoting the capacity of ovarian cancer cells to proliferate, migrate, and invade. Additionally, we have identified that genes co-expressed with APOC1 are primarily associated with adaptive immune responses. Notably, the levels of APOC1 in OV exhibit a correlation with the presence of M2 Tumor-associated Macrophages (TAMs). APOC1 emerges as a promising prognostic biomarker for OV and exhibits a significant association with M2 TAMs in OV.

中文翻译：

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APOC1 是与卵巢癌 M2 巨噬细胞相关的预后生物标志物

最近的研究表明APOC1与癌症进展相关，发挥促癌和免疫浸润促进作用。然而，目前还没有关于APOC1在卵巢癌（OV）中存在的报道。在本研究中，我们使用 GEO 和 TCGA 数据库进行数据分析。我们利用 cBioPortal、STRING、GeneMANIA、LinkedOmics、GSCALite、TIMER 和 CellMarker 等各种平台进行了彻底的生物信息学分析，以研究 APOC1 在 OV 中的功能。此外，我们对组织微阵列进行了免疫组织化学染色，并进行了体外细胞测定以验证我们的发现。我们的研究结果表明，与正常组织相比，OV 中 APOC1 的表达显着上调。重要的是，APOC1 水平高的患者预后明显较差。此外，我们的研究表明APOC1在促进卵巢癌细胞的增殖、迁移和侵袭能力方面发挥着至关重要的作用。此外，我们还发现与 APOC1 共表达的基因主要与适应性免疫反应相关。值得注意的是，OV 中的 APOC1 水平与 M2 肿瘤相关巨噬细胞 (TAM) 的存在相关。 APOC1 成为 OV 的一种有前景的预后生物标志物，并与 OV 中的 M2 TAM 显着相关。