Beet curly top disease is one of the most important viral diseases that causes economic damage to the yield of beet crops. The present study aimed to evaluate the effect of treating the Paya cultivar of sugar beet with Bacillus subtilis, Trichoderma harzianum, and Beta-aminobutyric acid (BABA), alone and in combination, on the growth and reaction of sugar beet to Beet curly top virus-Svr (BCTV-Svr). Anthocyanin concentration, peroxidase, and polyphenol oxidase enzyme activity were measured by spectrophotometry 14 days post-inoculation (dpi). At thirty dpi, virus accumulation was measured by quantitative polymerase chain reaction (qPCR), and physiological parameters in plants were assessed. The experiments were performed in a factorial, completely randomized design. The first symptoms of BCTV-Svr were observed at nine dpi in the control plants, while the symptoms appeared at 19 dpi in plants treated with B. subtilis, and between 12 and 14 dpi in the other treatments. Plants treated with B. subtilis and BABA showed the lowest and highest severity of the disease, respectively. The results of qPCR were consistent with the symptom severity results, and showed a significant difference in BCTV-Svr accumulation among the treatments. The highest level of anthocyanin concentration was observed in the B. subtilis treatment. On the other hand, polyphenol oxidase and peroxidase activity was increased using viral stress treatments at 14 dpi. The analyses showed that, in general, B. subtilis was more effective in inducing anthocyanin and enzyme activity in healthy and infected plants than the other treatments.

甜菜卷顶病是最重要的病毒性疾病之一，会对甜菜作物的产量造成经济损失。本研究旨在评估用枯草芽孢杆菌、哈茨木霉和β-氨基丁酸（BABA）单独或联合处理甜菜Paya品种对甜菜生长和对甜菜卷顶病毒的反应的影响-Svr（BCTV-Svr）。接种后 14 天 (dpi) 通过分光光度法测量花青素浓度、过氧化物酶和多酚氧化酶活性。在 30 dpi 时，通过定量聚合酶链式反应 (qPCR) 测量病毒积累，并评估植物的生理参数。实验以析因、完全随机的设计进行。在对照植物中，BCTV-Svr 的第一个症状在 9 dpi 时观察到，而在用枯草芽孢杆菌处理的植物中，症状在 19 dpi 时出现，而在其他处理中，则在 12 到 14 dpi 之间出现。用枯草芽孢杆菌和 BABA 处理的植物分别表现出最低和最高的疾病严重程度。 qPCR结果与症状严重程度结果一致，并且显示治疗间BCTV-Svr积累存在显着差异。在枯草芽孢杆菌处理中观察到最高水平的花青素浓度。另一方面，在 14 dpi 时使用病毒应激处理可提高多酚氧化酶和过氧化物酶活性。分析表明，总体而言，枯草芽孢杆菌在诱导健康和受感染植物中花青素和酶活性方面比其他处理更有效。