Abstract

Epithelial-to-mesenchymal transition (EMT) is a developmental program that plays a vital role in gastric cancer, including aspects of tumor progression, the metastatic process, and resistance to treatment. Here, we have designed an in vitro model that mimics the features of EMT as observed in gastric cancer. The results showed that both migration and invasion were enhanced in gastric cancer cells with Brachyury overexpression. Additionally, the expression of IL-8 increased, while IL-8RA and IL-8RB levels significantly decreased in the in vitro model. Overall, the in vitro model offers an opportunity to study these phenomena relevant to EMT as they may occur in vivo in gastric cancer, as well as potential drug interactions that could interfere with these processes.

中文翻译：

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胃癌上皮间质转化的体外模型设计

摘要

上皮间质转化（EMT）是一个在胃癌中发挥重要作用的发育程序，包括肿瘤进展、转移过程和治疗耐药等方面。在这里，我们设计了一个体外模型，模仿胃癌中观察到的 EMT 特征。结果表明，Brachyury 过表达的胃癌细胞的迁移和侵袭能力均增强。此外，在体外模型中，IL-8的表达增加，而IL-8RA和IL-8RB的水平显着降低。总体而言，体外模型提供了一个机会来研究这些与 EMT 相关的现象，因为它们可能在胃癌体内发生，以及可能干扰这些过程的潜在药物相互作用。