Recent studies have demonstrated the crucial role of Cytochrome P450 enzymes (CYPs) in the production of secondary metabolites, phytohormones and antioxidants in plants. However, their functional characterization specifically under alkaline stress remains elusive. CYP82C4 was the key gene screened from a family of wild soybean CYPs in our previous studies. The aim of this present study was to clone the Glycine soja GsCYP82C4 gene and characterize its functions in Arabidopsis and Glycine max. The results showed that the GsCYP82C4 gene displayed a high expression in different plant tissues at mature stages compared to young stages. Further, higher temporal expression of the GsCYP82C4 gene was noted at 6, 12 and 24 h time points after alkali treatment in leaves compared to roots. In addition, overexpression of GsCYP82C4 improved alkaline stress tolerance in Arabidopsis via increased root lengths and fresh biomass and strengthened the antioxidant defense system via a reduction in superoxide radicals in transgenic lines compared to wild type (WT) and atcyp82c4 mutants. Further, the expression levels of stress‐related marker genes were up‐regulated in GsCYP82C4 OX lines under alkali stress. The functional analysis of GsCYP82C4 overexpression in soybean displayed better hairy root growth, increased fresh weight, higher antioxidant enzyme activities and reduced lipid peroxidation rates in OX lines compared to the soybean WT (K599) line. In total, our study displayed positive roles of GsCYP82C4 overexpression in both Arabidopsis and Glycine max to alleviate alkaline stress via altering expression abundance of stress responsive genes, stronger roots, higher antioxidant enzyme activities as well as reduced rates of lipid peroxidation and superoxide radicals.

中文翻译：

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大豆细胞色素 P450 基因 GsCYP82C4 通过促进活性氧清除而赋予碱性耐受性

最近的研究表明，细胞色素 P450 酶 (CYP) 在植物次生代谢物、植物激素和抗氧化剂的产生中发挥着至关重要的作用。然而，它们在碱性胁迫下的功能特征仍然难以捉摸。CYP82C4是我们前期研究中从野生大豆CYP家族中筛选出的关键基因。本研究的目的是克隆大豆 GsCYP82C4基因并表征其在拟南芥中的功能最大甘氨酸结果表明，CYP82C4与年轻阶段相比，该基因在成熟阶段的不同植物组织中表现出高表达。此外，更高的时间表达CYP82C4与根相比，在碱处理后的 6、12 和 24 小时时间点，在叶子中注意到该基因。此外，过度表达CYP82C4与野生型 (WT) 和atcyp82c4突变体。此外，应激相关标记基因的表达水平上调CYP82C4碱胁迫下的 OX 系。的功能分析CYP82C4与大豆 WT (K599) 品系相比，在大豆中过表达 OX 品系表现出更好的毛状根生长、增加的鲜重、更高的抗氧化酶活性和降低的脂质过氧化率。总的来说，我们的研究显示了积极的作用CYP82C4在拟南芥和大豆通过改变应激反应基因的表达丰度、更强健的根系、更高的抗氧化酶活性以及降低脂质过氧化和超氧自由基的速率来减轻碱性应激。