Continuous glucose monitoring is very important to daily blood glucose control in diabetic patients, but its accuracy is limited by the narrow linear range of the response of biosensor to the glucose concentration because of the oxygen starvation in tissue and the limited maximum conversion rate of glucose oxidase. In this work, a biocompatible diffusion limiting membrane based on two medical-grade polyurethanes is developed via blending modification to restrict the diffusion flux of glucose to match the oxygen concentration and the maximum conversion rate. The expansiveness of the linear range for the nanomaterials-modified electrode in the glucose biosensor can be achieved through the regulation of two polyurethanes, the solvent, and the thickness of the membrane. In addition, the mass transport of hydrogen peroxide and interfering substances is also limited of the membrane. The experiments demonstrated that the membrane-modified microneedle biosensor exhibited a rapid response to the concentration variation of glucose, a wide linear range that is sufficient to cover the blood concentration of healthy and diabetic people, the ability to resist the oxygen concentration fluctuation and interfering substances, good reproducibility and long-term stability. The custom wearable electrochemical system, possessing these characteristics, has been proven to accurately monitor the blood concentration in a living rat in real time. This demonstrates a significant potential for application in both daily and clinical blood glucose monitoring.

中文翻译：

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用于具有宽线性范围的微针葡萄糖传感器的定制扩散限制膜

连续血糖监测对于糖尿病患者的日常血糖控制非常重要，但由于组织中的缺氧以及葡萄糖氧化酶的最大转化率有限，生物传感器对葡萄糖浓度的响应线性范围狭窄，其准确性受到限制。在这项工作中，通过共混改性开发了一种基于两种医用级聚氨酯的生物相容性扩散限制膜，以限制葡萄糖的扩散通量，以匹配氧气浓度和最大转化率。葡萄糖生物传感器中纳米材料修饰电极的线性范围的扩展可以通过调节两种聚氨酯、溶剂和膜的厚度来实现。此外，过氧化氢和干扰物质的传质也受到膜的限制。实验表明，膜修饰微针生物传感器对葡萄糖浓度变化具有快速响应能力，线性范围宽，足以覆盖健康人和糖尿病人的血液浓度，具有抵抗氧浓度波动和干扰物质的能力，良好的重现性和长期稳定性。定制的可穿戴电化学系统具有这些特性，已被证明可以准确地实时监测活体大鼠的血液浓度。这表明在日常和临床血糖监测中具有巨大的应用潜力。