In this work, a reusable DNA sensing microchip was developed for detection of vomitoxin (deoxynivalenol, DON) in sorghum using Cd-based core-shell CdSe@CdS quantum dots (QDs) as promising electrochemiluminescence (ECL) emitter. The size-adjustable aqueous phase CdSe@CdS QDs were prepared through homogeneous method, exhibiting strong cathodic ECL emission with a central wavelength of 520 nm in SO coreactant. And gold nanoparticles-modified iron cobalt cyanide hydrate (Fe–Co–Au) was introduced as an accelerator to amplify the ECL signal. ECL signal was quenched after the formation of a double-stranded (dsDNA) S1–S2 by generating an electron transfer system between the emitter and ferrocene (Fc), which are modified on the aptamer (ssDNA S1) and its complement sequence (ssDNA S2), respectively. When the target DON is presence, the aptamer ssDNA S1 will bind to the DON and trigger the unbinding of double strands DNA and the release of the ssDNA S2, thus the signal can be generated. This approach offers a feasible method for the detection of DON within the range of 1 ng/mL to 200 ng/mL.

中文翻译：

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可重复使用电化学发光传感微芯片检测呕吐毒素的研制

在这项工作中，开发了一种可重复使用的DNA传感微芯片，使用基于镉的核壳CdSe@CdS量子点（QD）作为有前途的电化学发光（ECL）发射器来检测高粱中的呕吐毒素（脱氧雪腐镰刀菌烯醇，DON）。通过均相法制备了尺寸可调的水相CdSe@CdS量子点，在SO共反应物中表现出中心波长为520 nm的强阴极ECL发射。并引入金纳米粒子修饰的铁钴氰化物水合物（Fe-Co-Au）作为加速剂来放大 ECL 信号。通过在发射器和二茂铁 (Fc) 之间生成电子转移系统，形成双链 (dsDNA) S1–S2 后，ECL 信号被淬灭，该系统在适体 (ssDNA S1) 及其互补序列 (ssDNA S2) 上进行修饰）， 分别。当目标DON存在时，适配体ssDNA S1会与DON结合并触发双链DNA的解开和ssDNA S2的释放，从而产生信号。该方法为检测1 ng/mL至200 ng/mL范围内的DON提供了可行的方法。