In this study, we report a new carbazole-malononitrile fluorescent probe with an interesting aggregation-induced emission (AIE) characteristic. Probe could rapidly and selectively detect hydrazine (NH) in ～100% aqueous media, and also exhibit an exceedingly low detection limit of 6.3 nM for sensitively detecting NH. The sensing mechanism of towards NH has been well demonstrated through the spectra of H NMR, HRMS and FTIR. Interestingly, probe was applied to visualize and detect gaseous and aqueous NH with sensitive color changes. Importantly, probe was applied to effectively detect NH in practical samples such as soil, human serum, human urine, plants, foods and beverages, as well as sensitively sense and image NH in biological systems including living mungbean sprouts, , and HeLa cells.

中文翻译：

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一种具有 AIE 特性的新型咔唑荧光探针，用于活细胞、绿豆芽、拟南芥和实际样品中肼的检测和成像

在这项研究中，我们报告了一种新的咔唑-丙二腈荧光探针，具有有趣的聚集诱导发射（AIE）特性。探针可以快速、选择性地检测～100%水介质中的肼（NH），并且还表现出极低的检测限（6.3 nM），可灵敏地检测NH。通过 1H NMR、HRMS 和 FTIR 光谱很好地证明了对 NH 的传感机制。有趣的是，探针用于可视化和检测具有敏感颜色变化的气态和水态 NH。重要的是，该探针可用于有效检测土壤、人血清、人尿液、植物、食品和饮料等实际样品中的NH，以及对活体绿豆芽、细胞和HeLa细胞等生物系统中的NH进行灵敏感知和成像。