A phytochemical investigation of Kaempferia champasakensis rhizomes led to the isolation of a new 3,4-seco-isopimarane diterpene, kaempferiol A (1), and three new isopimarane diterpenes, kaempferiols B–D (2–4), together with six known isopimarane diterpenes (5–10). The structures of 1–4 were elucidated by extensive spectroscopic analyses, including HR-ESI–MS, UV, IR, and 1D and 2D NMR. The absolute configurations of 1, 3, and 4 were determined by ECD calculations, while that of 2 was established using the modified Mosher method. All isolated compounds were tested for cytotoxicity against three human cancer cell lines, lung cancer (A549), cervical cancer (HeLa), and breast cancer (MCF-7). Among them, 6 and 7 showed moderate cytotoxic activities against the three tested cell lines, with IC₅₀ values ranging from 38.04 to 27.77 μM, respectively.

Graphical abstract

中文翻译：

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越南产山柰中的一种新的3,4-仲异海松烷和三种新的异海松烷二萜及其细胞毒活性

对占巴沙肯西斯山柰根茎的植物化学研究分离出一种新的 3,4-seco-异海松烷二萜，山奈醇 A ( 1 ) 和三种新的异海松烷二萜，山奈醇 B–D ( 2 – 4)，以及六种已知的异海松烷二萜 ( 5–10)。通过广泛的光谱分析（包括 HR-ESI-MS、UV、IR、1D 和 2D NMR）阐明了1 – 4的结构。 1、3和4的绝对构型是通过ECD计算确定的，而2的绝对构型是使用改进的Mosher方法建立的。所有分离的化合物均针对三种人类癌细胞系（肺癌（A549）、宫颈癌（HeLa）和乳腺癌（MCF-7））进行了细胞毒性测试。其中，6和7对三种测试细胞系均表现出中等的细胞毒活性，IC ₅₀值分别为38.04至27.77 μM。

图形概要