Ligand-protected metal clusters are essential in optics, catalysis, and biomedicine due to their unique electronic transfers and rapid renal clearance, but their stability and photostability are still highly challenged. Here, we develop atomically precise Ag₂₉ clusters with 900–1,200 nm emissions that exhibit ultrahigh structural stability for 2 months and distinct photostability for 140 min via Pt single-atom engineering. The density functional theory shows that Ag₂₉ behaves as a molecular-like semiconductor with a direct band gap of 1.3 eV. Meanwhile, the Pt single atom introduces rich 5d states in the highest occupied molecular orbital, leading to a continuous electron supply that ensures high photostability. We acquire numerous images of blood vessels, which enables machine learning to predict high-resolution near-infrared II (NIR-II) images. The NIR-II light sheet microscopy achieves a large field of view and deep-tissue imaging without photobleaching and accurately surveils the pathological progression of intestinal injury at the depth of 2.4 μm.

配体保护的金属簇由于其独特的电子转移和快速的肾脏清除而在光学、催化和生物医学中至关重要，但其稳定性和光稳定性仍然受到高度挑战。在这里，我们开发了原子级精确的 Ag ₂₉簇，具有 900-1,200 nm 的发射波长，通过 Pt 单原子工程表现出 2 个月的超高结构稳定性和 140 分钟的独特光稳定性。密度泛函理论表明Ag ₂₉表现为类分子半导体，直接带隙为1.3 eV。同时，Pt单原子在最高占据分子轨道中引入丰富的5d态，从而产生连续的电子供应，确保高光稳定性。我们获取了大量血管图像，这使得机器学习能够预测高分辨率近红外 II (NIR-II) 图像。 NIR-II光片显微镜可实现大视场和深层组织成像，无需光漂白，可准确监测2.4μm深度肠道损伤的病理进展。