Deadwood-inhabiting bacteria and fungi were crucial in understanding ecosystem functioning in carbon turnover. The objectives of this study were to investigate microbial succession in deadwood and analyze bacterial-fungal ratio, composition, and diversity of various tree species; it explored fungal traits during decomposition and uncovered unique bacterial-fungal networks; thinning effects on microbial composition over three years and the roles of nitrogen cycle in energy metabolism were assessed; the analysis included carbohydrate-active enzyme gene families, examining bacterial and fungal contributions to organic compound degradation; the impacts of thinning degrees on specific carbohydrate-active enzyme gene families were also evaluated. Deadwood samples of and in decomposition time series spanning 2017, 2018, and 2019 under thinning degrees of 0%, 20%, 40%, 60%, and 80% were analyzed using metagenomics. The results reflected the decomposition stages of deadwood with changes in microbial composition and function over time. Furthermore, this study revealed the dominance of microbial groups, including and for bacteria and Ascomycota and Basidiomycota for fungi. This study revealed the prevalence of wood saprotrophs and mycoparasites. Deadwood of fostered more microbial networks with mutualistic relationships, while encouraged more microbial networks with antagonistic interactions. The sensitivity of microbial composition to thinning degrees was primarily evident at lower taxonomic levels (such as family to species). Furthermore, nitrogen cycle genes and carbohydrate-active enzyme gene families unveiled the roles of bacteria and fungi, including nitrogen fixation and lignin degradation. Thinning significantly impacted the gene families of carbohydrate-active enzymes, especially in the second year, influencing the microbial resource requirements and metabolic function in deadwood decomposition. In conclusion, the findings provided insights into the intricate microbial dynamics and their ecological implications in tropical forests. Lastly, this study underscored the importance of actively managing sustainable forests to promote desired microbial roles, thus enhancing ecosystem functioning.

中文翻译：

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森林疏伐对各种树种的微生物组成和功能以及多重分解时间序列的级联效应：热带森林枯木分解的见解

栖息在枯枝上的细菌和真菌对于理解生态系统在碳周转中的功能至关重要。本研究的目的是调查枯木中的微生物演替，并分析各种树种的细菌-真菌比率、组成和多样性；它探索了分解过程中的真菌特征，并发现了独特的细菌-真菌网络；评估了三年内对微生物组成的稀疏影响以及氮循环在能量代谢中的作用；该分析包括碳水化合物活性酶基因家族，检查细菌和真菌对有机化合物降解的贡献；还评估了稀疏程度对特定碳水化合物活性酶基因家族的影响。使用宏基因组学分析了 2017 年、2018 年和 2019 年间伐程度为 0%、20%、40%、60% 和 80% 的枯木样本。结果反映了枯木的分解阶段以及微生物组成和功能随时间的变化。此外，这项研究揭示了微生物群体的优势，包括细菌和子囊菌门以及真菌的担子菌门。这项研究揭示了木材腐生菌和真菌寄生虫的流行。戴德伍德培育了更多具有互利关系的微生物网络，同时鼓励了更多具有对抗性相互作用的微生物网络。微生物组成对稀疏程度的敏感性主要在较低的分类水平（例如科到物种）上明显。此外，氮循环基因和碳水化合物活性酶基因家族揭示了细菌和真菌的作用，包括固氮和木质素降解。间伐显着影响了碳水化合物活性酶的基因家族，尤其是在第二年，影响了枯木分解中的微生物资源需求和代谢功能。总之，这些发现为热带森林中复杂的微生物动态及其生态影响提供了见解。最后，这项研究强调了积极管理可持续森林以促进所需微生物作用的重要性，从而增强生态系统功能。