Developing anticancer drugs is a complex and time-consuming process. The inability of current laboratory models to reflect important aspects of the tumor in vivo limits anticancer medication research. Zebrafish is a rapid, semi-automated in vivo screening platform that enables the use of non-invasive imaging methods to monitor morphology, survival, developmental status, response to drugs, locomotion, or other behaviors. Zebrafish models are widely used in drug discovery and development for anticancer drugs, especially in conjunction with live imaging techniques. Herein, we concentrated on the use of zebrafish live imaging in anticancer therapeutic research, including drug screening, efficacy assessment, toxicity assessment, and mechanism studies. Zebrafish live imaging techniques have been used in numerous studies, but this is the first time that these techniques have been comprehensively summarized and compared side by side. Finally, we discuss the hypothesis of Zebrafish Composite Model, which may provide future directions for zebrafish imaging in the field of cancer research.

开发抗癌药物是一个复杂且耗时的过程。当前的实验室模型无法反映体内肿瘤的重要方面，限制了抗癌药物研究。斑马鱼是一种快速、半自动化的体内筛选平台，可以使用非侵入性成像方法来监测形态、存活、发育状态、对药物的反应、运动或其他行为。斑马鱼模型广泛应用于抗癌药物的药物发现和开发，特别是与实时成像技术结合使用。在此，我们重点关注斑马鱼活体成像在抗癌治疗研究中的应用，包括药物筛选、疗效评估、毒性评估和机制研究。斑马鱼活体成像技术已被用于许多研究中，但这是第一次对这些技术进行全面总结和并排比较。最后，我们讨论了斑马鱼复合模型的假设，该模型可能为斑马鱼成像在癌症研究领域提供未来的方向。