Thirty polymorphic microsatellites were developed and characterised for the aquaculture important species, Green chromide, Etroplus suratensis through Illumina Sequencing with a mean read quality (Phred score) of 35.8 and the microsatellite primers were designed from the sequenced information, considering various attributes such as the number of repeats, Tm, GC content, Product size, etc. using the software PRIMER 3.0 web tool. The allele numbers ranged from 6 to 18. The mean observed heterozygosity was 0.745 and the mean expected heterozygosity was 0.762. The average PIC value for 30 microsatellite primers was 0.80 and all the loci had PIC values > 0.75. The identified polymorphic loci would help in revealing the genetic stock identification among the Pearlspot population.

通过 Illumina 测序，开发并表征了水产养殖重要物种 Green chromide、Etroplus suratensis 的30 个多态性微卫星，平均读数质量（Phred 评分）为 35.8，并根据测序信息设计了微卫星引物，考虑了数量等各种属性。使用软件 PRIMER 3.0 网络工具测量重复次数、Tm、GC 含量、产品尺寸等。等位基因数范围为 6 至 18。观察到的平均杂合度为 0.745，平均预期杂合度为 0.762。 30 个微卫星引物的平均 PIC 值为 0.80，所有位点的 PIC 值都 > 0.75。鉴定出的多态性位点将有助于揭示珍珠斑种群的遗传种群鉴定。